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Z 4 hydroxytamoxifen

Manufactured by Santa Cruz Biotechnology

The (Z)-4-hydroxytamoxifen is a research-use chemical compound. It is a selective estrogen receptor modulator (SERM) that can bind to and modulate the activity of estrogen receptors. This compound is commonly used in research applications to study the role of estrogen receptors in various biological processes.

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4 protocols using z 4 hydroxytamoxifen

1

Tamoxifen Compound Preparation Protocol

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(Z)-4-hydroxyTamoxifen (Santa Cruz SC-3542) was dissolved in ethanol and added to the media at a final concentration of 1μM. Tamoxifen (Sigma T5648) was dissolved in corn oil to a final concentration of 30 g mL−1.
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2

Tamoxifen and PMA Stimulation Assay

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(Z)-4-hydroxyTamoxifen (Santa Cruz Biotechnology, SC-3542) was dissolved in ethanol and added to the cell culture media at a final concentration of 1 μmol/L. Tamoxifen (Sigma T5648) was dissolved in corn oil to a final concentration of 30 g/mL. Phorbol 12-myristate 13-acetate (PMA) (P1585), ionomycin (I3909) and Brefeldin A (B7651) were purchased from Sigma.
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3

Tamoxifen and PMA Stimulation Assay

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(Z)-4-hydroxyTamoxifen (Santa Cruz Biotechnology, SC-3542) was dissolved in ethanol and added to the cell culture media at a final concentration of 1 μmol/L. Tamoxifen (Sigma T5648) was dissolved in corn oil to a final concentration of 30 g/mL. Phorbol 12-myristate 13-acetate (PMA) (P1585), ionomycin (I3909) and Brefeldin A (B7651) were purchased from Sigma.
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4

Quantification of Tamoxifen Metabolites

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Tamoxifen, (Z)-Endoxifen, and Propranolol Hydrochloride were purchased from Sigma-Aldrich. (Z)-4-HydroxyTamoxifen and N-desmethylTamoxifen were purchased from Santa Cruz Biotechnology. Acetonitrile HPLC grade, Formic acid as eluent and methanol HPLC grade were obtained from Merck. VAMS Mitra® tips was purchased from Mitra Neoteryx, USA.
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