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Mab 1914p

Manufactured by Vector Laboratories
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The MAB-1914P is a mouse monoclonal antibody produced by Vector Laboratories. It is designed for use in immunohistochemical and other immunoassay applications.

Automatically generated - may contain errors

Lab products found in correlation

Blocking buffer, by Roche (2 mentions) Nb100 304, by Novus Biologicals (2 mentions) Dl 1207, by Vector Laboratories (2 mentions) Ab5539, by Merck Group (2 mentions) Sc 188, by Alomone (2 mentions) Acc 030, by Novus Biologicals (2 mentions) Nbp1 49461, by Novus Biologicals (2 mentions) Eclipse 80i microscope, by Nikon (2 mentions)

2 protocols using mab 1914p

1

Dorsal Root Ganglion and Spinal Cord Analysis

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Dorsal root ganglia (DRG) and spinal cords were harvested from injured mAtf3pro/RmGFP reporter mice perfused with ice-cold PBS followed by cold 4% paraformaldehyde (PFA). In addition, sciatic nerves were harvested distal to the crush in treated mAtf3pro/RmGFP reporter mice perfused with cold 4% PFA. Perfused tissues were post-fixed for 3 hr at 4°C, and cryoprotected with 30% sucrose in PBS overnight. DRG sections (10 μm), spinal cord sections (20 μm) and sciatic nerve sections (60 μm) collected, blocked and permeabilized with 1% Triton X-100 in blocking buffer (Roche Diagnostics) for 1 hr at RT. Sections were incubated with rabbit polyclonal antibody against ATF3 (Santa Cruz Biotech; sc-188; 1:1000), TRPV1 (Alomone; ACC-030; 1:1000), SCG10 (Novus; NBP1-49461; 1:2000), 53BP1 (Novus; NB100-304; 1:2000), chicken polyclonal antibody against NF200 (Millipore, AB5539; 1:2000), rat polyclonal antibody against Laminin-γ (Millipore; MAB-1914P; 1:1000), DyLignt_594 conjugated Isolectin B4 (VectorLab; DL-1207; 1: 200), or mouse monoclonal antibody against phospho-Histone H2A.X (Ser139)(γ-H2AX; gamma-H2AX) (Millipore; 05-636; 1:400) at 4°C overnight and then incubated with Alexa Fluor 568 goat antibody against rabbit IgG, chicken IgG or rat IgG for 1 hr at RT. Images were acquired using a Nikon Eclipse 80I Microscope.
Cheng Y.C., Snavely A., Barrett L.B., Zhang X., Herman C., Frost D.J., Riva P., Tochitsky I., Kawaguchi R., Singh B., Ivanis J., Huebner E.A., Arvanites A., Oza V., Davidow L., Maeda R., Sakuma M., Grantham A., Wang Q., Chang A.N., Pfaff K., Costigan M., Coppola G., Rubin L.L., Schwer B., Alt F.W, & Woolf C.J. (2021). Topoisomerase I inhibition and peripheral nerve injury induce DNA breaks and ATF3-associated axon regeneration in sensory neurons. Cell reports, 36(10), 109666.
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2

Dorsal Root Ganglion and Spinal Cord Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Dorsal root ganglia (DRG) and spinal cords were harvested from injured mAtf3pro/RmGFP reporter mice perfused with ice-cold PBS followed by cold 4% paraformaldehyde (PFA). In addition, sciatic nerves were harvested distal to the crush in treated mAtf3pro/RmGFP reporter mice perfused with cold 4% PFA. Perfused tissues were post-fixed for 3 hr at 4°C, and cryoprotected with 30% sucrose in PBS overnight. DRG sections (10 μm), spinal cord sections (20 μm) and sciatic nerve sections (60 μm) collected, blocked and permeabilized with 1% Triton X-100 in blocking buffer (Roche Diagnostics) for 1 hr at RT. Sections were incubated with rabbit polyclonal antibody against ATF3 (Santa Cruz Biotech; sc-188; 1:1000), TRPV1 (Alomone; ACC-030; 1:1000), SCG10 (Novus; NBP1-49461; 1:2000), 53BP1 (Novus; NB100-304; 1:2000), chicken polyclonal antibody against NF200 (Millipore, AB5539; 1:2000), rat polyclonal antibody against Laminin-γ (Millipore; MAB-1914P; 1:1000), DyLignt_594 conjugated Isolectin B4 (VectorLab; DL-1207; 1: 200), or mouse monoclonal antibody against phospho-Histone H2A.X (Ser139)(γ-H2AX; gamma-H2AX) (Millipore; 05-636; 1:400) at 4°C overnight and then incubated with Alexa Fluor 568 goat antibody against rabbit IgG, chicken IgG or rat IgG for 1 hr at RT. Images were acquired using a Nikon Eclipse 80I Microscope.
Cheng Y.C., Snavely A., Barrett L.B., Zhang X., Herman C., Frost D.J., Riva P., Tochitsky I., Kawaguchi R., Singh B., Ivanis J., Huebner E.A., Arvanites A., Oza V., Davidow L., Maeda R., Sakuma M., Grantham A., Wang Q., Chang A.N., Pfaff K., Costigan M., Coppola G., Rubin L.L., Schwer B., Alt F.W, & Woolf C.J. (2021). Topoisomerase I inhibition and peripheral nerve injury induce DNA breaks and ATF3-associated axon regeneration in sensory neurons. Cell reports, 36(10), 109666.
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