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7aad and pe

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7AAD and PE are fluorescent dyes used in flow cytometry applications. 7AAD is a DNA-binding dye that can be used to detect dead or late apoptotic cells. PE is a fluorescent protein that can be used to label and detect specific cell surface markers or proteins. These dyes provide tools for cell analysis and identification.

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Lab products found in correlation

Facscalibur, by BD (1 mentions) Flow cytometry, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

7AAD and PE-labeled Annexin V (2 citations) PE Annexin V (556421) and 7AAD (21-68981E) (2 citations)

3 protocols using 7aad and pe

1

Cell Cycle and Apoptosis Analysis

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ESCC cell samples were reaped and fixed with 4% formaldehyde on ice for 1 h, then mixed with RNase for 30 min. For cycle analysis, samples were cultured with Propidium iodide (PI), then subjected to a flow cytometer using FACSCalibur (BD Biosciences, San Jose, CA, USA). The percentages of a cell sample in G0/G1, S, and G2/M phases were measured. For apoptosis analysis, samples were double-stained for 15 min with Annexin V-labeled with 7AAD and PE (BD Biosciences), finally exposed to flow cytometer.
Li Z.W., Zhang T.Y., Yue G.J., Tian X., Wu J.Z., Feng G.Y, & Wang Y.S. (2020). Small nucleolar RNA host gene 22 (SNHG22) promotes the progression of esophageal squamous cell carcinoma by miR-429/SESN3 axis. Annals of Translational Medicine, 8(16), 1007.
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2

Annexin V and 7AAD Apoptosis Assay

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Collected cells were resuspended in 100 μl of 1 × buffer with fluorescein isothiocyanate (FITC)‐labelled annexin V and PE or 7AAD and PE (5 μl each, BD) and incubated for 15 min in the dark. Thereafter, cells were added with buffer (400 μl) and flow cytometry was carried out within 1 h to determine the rate of cell apoptosis.
Chen S., Li Q., Chen X., Bao H., Wu W., Shen F., Lu B., Jiang R., Zong Z, & Zhao Y. (2022). SNORA70E promotes the occurrence and development of ovarian cancer through pseudouridylation modification of RAP1B and alternative splicing of PARPBP. Journal of Cellular and Molecular Medicine, 26(20), 5150-5164.
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3

Apoptosis Quantification by Flow Cytometry

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After being cultured at 6-well plates for 48 h, cells were fixed in 70% ethanol pre-cooled with ice for 2 h. Quantification of apoptosis was measured by flow cytometry (Thermo Fisher Scientific) after staining with Annexin V-labeled with 7AAD and PE (BD Biosciences, San Jose, CA, USA).
Wu G., Hao C., Qi X., Nie J., Zhou W., Huang J, & He Q. (2020). LncRNA SNHG17 aggravated prostate cancer progression through regulating its homolog SNORA71B via a positive feedback loop. Cell Death & Disease, 11(5), 393.
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