Ethyl bromopyruvate (technical grade, 90%), thiourea (ACS reagent) benzaldehyde (ReagentPlus® 99%), salicylaldehyde (reagent grade, 98%), 3-hydroxybenzaldehyde (AR ≥ 99%), 4-hydroxybenzaldehyde (Analytical standard), 4-hydroxy-3-methoxybenzaldehyde (ReagentPlus® 99%), acetophenone (Analytical standard), 2′-hydroxyacetophenone (ReagentPlus® 99%),. Ethanol (absolute, ACS reagent) and glacial acetic acid (100%, anhydrous for analysis ACS, ISO reagent) were used purchased from Sigma Aldrich and Merck. Synthesized compounds were purified by recrystallization in appropriate solvents and examined through thin layer chromatography (Merck Silica gel 60 F254). Melting points were determined by using digital Gallenkamp model MPD BM 3.5 apparatus. Characterization of synthesized compounds was made through spectrophotometric analysis; FT-IR (Thermoscientific NICOLET IS10 spectrophotometer), 1H & 13C NMR (Bruker AM-300 and AM-100 spectrophotometer) using DMSO and CDCl3 respectively. Elemental analysis values were recorded on Model ANALYST 2000 CHNS, Perkin Elmer Analyzer. Multiskan™ GO Microplate Spectrophotometer was used to quantify synthesized compounds for accuracy, precision and sensitivity [26 ].
Nicolet is10 spectrophotometer
Manufactured by Bruker
The NICOLET IS10 spectrophotometer is a laboratory instrument designed to identify and analyze the molecular composition of materials. It measures the absorption, emission, or scattering of infrared light by a sample, providing information about the sample's chemical structure and properties.
Automatically generated - may contain errors
Lab products found in correlation
Merck silica gel 60 f254, by Merck Group (1 mentions)
Multiskan go microplate spectrophotometer, by MultiSciences Biotech (1 mentions)
Glacial acetic acid, by Merck Group (1 mentions)
Am 300, by Bruker (1 mentions)
Sec malls, by Wyatt Technology (1 mentions)
Am 300 spectrophotometer, by Bruker (1 mentions)
3 protocols using nicolet is10 spectrophotometer
1
Synthesis of Substituted Benzaldehyde Derivatives
2
Structural Characterization of Polymer Blends
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1H NMR and 13C NMR were recorded with Bruker 400 MHz using CDCl3 as the solvent. The ratio of 1,4- and 1,2-unit content of PB, and ratio of cis-1,4 and 3,4-unit content of PI were determined by 1H NMR, and the ratio of cis-1,4 and trans-1,4 unit content of PB was determined by 13C NMR.21–24 (link) In addition, the actual ratio of the PS and PB/PI segments in the copolymer was also estimated by 1H NMR, according to a previously published method.22–25 (link) FT-IR spectra were measured using a Bruker Nicolet iS10 spectrophotometer. The number-average molecular weights (Mn) and dispersity (Mw/Mn) of the polymers were measured by a DAWN EOS size exclusion chromatography/multi-angle light scatter instrument (SEC-MALLS, Wyatt Technology), the polymer samples were dissolved in HPLC grade THF with the concentration of 10.0 mg ml−1, flow rate of eluent was 0.5 ml min−1.
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1H NMR and 13C NMR were recorded with Bruker 400 MHz using CDCl3 as the solvent. The ratio of 1,4- and 1,2-unit content of PB, and ratio of cis-1,4 and 3,4-unit content of PI were determined by 1H NMR, and the ratio of cis-1,4 and trans-1,4 unit content of PB was determined by 13C NMR.21–24 (link) In addition, the actual ratio of the PS and PB/PI segments in the copolymer was also estimated by 1H NMR, according to a previously published method.22–25 (link) FT-IR spectra were measured using a Bruker Nicolet iS10 spectrophotometer. The number-average molecular weights (Mn) and dispersity (Mw/Mn) of the polymers were measured by a DAWN EOS size exclusion chromatography/multi-angle light scatter instrument (SEC-MALLS, Wyatt Technology), the polymer samples were dissolved in HPLC grade THF with the concentration of 10.0 mg ml−1, flow rate of eluent was 0.5 ml min−1.
3
Compound Purification and Characterization
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The purification of all synthesized compounds was accomplished through recrystallization using an appropriate solvent. The purity of the compounds was subsequently confirmed via thin-layer chromatography on silica gel F254. Characterization of the synthesized compounds was conducted through spectrophotometric analyses, specifically Fourier-transform infrared spectroscopy (FTIR) using a Thermoscientific NICOLET IS10 Spectrophotometer, and proton nuclear magnetic resonance (1H NMR) using a Bruker AM-300 Spectrophotometer. DMSO and chloroform were employed as solvents in these analyses.
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