The EX Taq DNA polymerase Hot Start Version kit is a DNA polymerase designed for PCR amplification. It is a thermostable DNA polymerase derived from Thermus aquaticus with a hot-start feature that helps to improve specificity and sensitivity.
Example 1
In one example, a PCR reaction was conducted using a Takara EX Taq DNA polymerase Hot Start Version kit (Takara, cat no. RR006A) according to manufacture instructions. A potential bacterial DNA sample and primer pair SEQ ID NOs: 1 and 2 were used to produce an amplicon. This primer pair amplified a segment of the 16S gene. The results of the reaction are provided in
Example 2
PCR reaction was conducted using a Takara EX Taq DNA polymerase Hot Start Version kit (Takara, cat no. RR006A) according to manufacture instructions. The sample and primer pair SEQ ID NOs: 3 and 4 were used to produce an amplicon. This primer pair amplified a segment of the 16S rRNA. The reaction also involved the use of reverse transcriptase. The results of the reaction are provided in
PCR Reaction Mixture:
The master mixes evaluated consisted of the reference mastermix [ 1/10th volume 10× Ex Taq Buffer (proprietary mix), 0.2 mM of each dNTP, 1.25 units of Ex Taq, 0.2 mM each of primers and probe, template and water up to a final volume of 25 uls. which was supplemented with the different probes. The reference mastermix was prepared in a large batch.
PCR Reaction:
The following thermal cycling steps were applied:
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