Evo40 scanning

Each strain was grown as a biofilm on 10-mm diameter silicone disks in CDMB for 5 days under static conditions at 32 °C for examination by scanning electron microscopy (SEM). After removal of the medium, biofilms were fixed in 2% glutaraldehyde, followed by 1% osmium tetraoxide for 30 minutes each. The samples were dehydrated in a sequential series of ethanol solutions (increasing from 10% to 100%). The disks were critical point-dried, sputter-coated with carbon, and examined using a Zeiss EVO40 scanning electron microscope.

The morphology of the pristine PVC electrospun fibers and PVC fibers with encapsulated enzyme was observed using an EVO40 scanning electron microscope (SEM, Zeiss, Jena, Germany).
The results obtained from spectrophotometric measurements obtained using V-750 UV-Vis spectrophotometer (Jasco, Tokyo, Japan) allowed us to evaluate the changes in the concentration of the ABTS (λ = 420 nm), SMX (λ = 257 nm) and CBZ (λ = 284) during conversion processes. The concentrations of SMX and CBZ were determined using a calibration curve for each compound. The pharmaceuticals removal efficiency (RE (%)) was determined by using the following Equation (2): $$RE\left(\%\right)=\frac{C_B-C_A}{C_B}·100\%$$
where C_B and C_A denote pharmaceutical concentration before and after removal, respectively.