MPM-334-1 was scanned using a GE Phoenix Nanotom M at the Molecular Imaging Center of the University of Southern California (USC). MPM-334-1 was scanned at 9.99 µm voxel size, 125 kV, 200 mA, exposure time 750.36 ms, averaging two frames and skipping one frame, 360 degrees rotation 1440 frames and 0.1 mm Cu + Cu filter. The scans were initially reconstructed using GE phoenix datos|×2 2.3.3.160. The three-dimensional reconstruction of the skull was generated in Avizo Lite (9.2). Digital mesh cleaning was conducted using Geomagic (2013). Final imaging of the volumes was conducted using Blender and Avizo Lite (9.2).
Phoenix nanotom m
Manufactured by GE Healthcare
Sourced in United States, Germany
The Phoenix Nanotom M is a high-resolution computed tomography (CT) system designed for non-destructive 3D imaging of a wide range of samples. It provides high-quality, high-resolution scans with a focus on nanoscale detail.
Automatically generated - may contain errors
Lab products found in correlation
8 protocols using phoenix nanotom m
1
High-Resolution 3D Skull Imaging Protocol
2
Multi-Modal Imaging of Materials
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Scanning electron microscopy (SEM) images were performed using JEOL JSM-7100F microscope operated at 20 keV. Laser scanning microscope (LSM) images LSM (VK-X100 series LSM 3D Profile Measurement from KEYENCE) was employed for optical observations. X-ray micro computed tomography (µCT) scans were performed using GE’s phoenix nanotom m (now distributed by BarkerHughes, a GE Company) at a focal spot size of 1.7 µm. All scans were done at a tube voltage of 80 kV and 110 µA tube current. The detector of the manotom m (GE’s DXR500L) features 3070 × 2400 pixel, 100 µm pixel pitch size, and a dynamic range of 10.000:1. Full rotation scans with 1600 projections, an average of 3 images per angular increment and an exposure time of 1000 ms per image yielded a total scan duration of 1 h 40 min. The cone beam geometry resulted in a geometrical magnification factor of 66.7 and a voxel size of 1.5 × 1.5 × 1.5 µm3. Volume reconstruction was done with the commercially available phoenix datos|x software package (BakerHughes, a GE Company). For the reduction of image noise, the 3D image data was post processed using a median filter with a kernel size of 5 voxels.
3
Microstructure analysis of fossil dentitions
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MPM-90, MPM-373, and MPM-351 were scanned using a GE Phoenix Nanotom M at the Molecular Imaging Center of the University of Southern California (USC). MPM-90 and MPM-351 were scanned at 2.73 µm voxel size, 80 kV, 100 mA, exposure time 1250 ms, averaging two frames and skip one frame, 360° rotation 1000 frames, no filter. All three specimens were scanned at 10 µm voxel size, 100 kV, 200 mA, exposure time 750 ms, averaging three frames and skip one frame, 360° rotation 1440 frames, 0.1 mm Cu filter. The scans were reconstructed through GE phoenix datos| × 2 reconstruction 2.3.3.160. The three-dimensional visualization and analyses are conducted using Avizo Lite (9.2). The dentitions were also segmented and measured by tools of this software. The segmentation of MPM-90 and MPM-351 was conducted using the scans at 2.73 µm resolution.
4
Compressive Strength of Sedge Nutlets
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Small sedge nutlets were compressed between flat plates at speeds of 1–2 mm min−1 and the resultant force and displacements recorded by a materials tester (FLS-1 tester, Lucas Scientific, New York, USA) fitted with a 2 kN load cell. CT images of intact seeds were made on a GE Phoenix Nanotom M (Wunstorf, Germany). Scans were displayed at a resolution of 0.93 µm/voxel using a voltage of 100 kV at a current of 260 µA. Total scan time was 250 min.
5
Contrast-Enhanced Nano-CT Imaging of Vascular Network
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Ten out of 16 euthanized mice per group were infused via the left ventricle with a barium sulfate solution, an X-ray contrasting agent, to fill up blood vessel according to a method validated in our team [35 (link)]. Following the infusion, the right knee was obtained by cutting half the femur and tibia/fibula above and below the joint line. Excess muscles were removed, and joints were fixed in 4% PFA (48h at 4°C), embedded undecalcified with knee in extension in methyl-methacrylate (MMA) resin for nano-CT and histology analysis.
A standardized parallel-beam nano-CT scan was performed within a field of view of 7.2mm height and 5mm width using a nano-CT (GE Phoenix Nanotom℗ m, Wunstorf, Germany). Scans were conducted at 90 kV, 120 μA with a 750 ms exposure time and a rotation step of 0.15°. The source-to-sample distance was 15 mm and the source-to-detector distance was 250 mm, leading to an effective pixel size of 3 μm. The scanned region included all tissues between distal femoral epiphysis up to proximal tibia including the metaphysis. An XY image (1307 * 1228 pixels) stack was obtained. As seen inFig 1a and 1c , structures appearing in bright white represent the vascular network due to the high contrasting power of barium sulfate, while the bone appeared gray and the marrow in black (non-contrasted).
A standardized parallel-beam nano-CT scan was performed within a field of view of 7.2mm height and 5mm width using a nano-CT (GE Phoenix Nanotom℗ m, Wunstorf, Germany). Scans were conducted at 90 kV, 120 μA with a 750 ms exposure time and a rotation step of 0.15°. The source-to-sample distance was 15 mm and the source-to-detector distance was 250 mm, leading to an effective pixel size of 3 μm. The scanned region included all tissues between distal femoral epiphysis up to proximal tibia including the metaphysis. An XY image (1307 * 1228 pixels) stack was obtained. As seen in
6
Micro-CT Analysis of Scaffold Pore Structure
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Scaffold and bone specimens were scanned in cross section at 10 μm voxel resolution and reconstructed into 3d images on a Scanco 80 micro-CT system (Scanco USA, Wayne, PA, USA). Images were inverted to visualize the porous domains so pore size and morphology could be quantified using methods previously described by Lin et al. [59 (link)]. Additional specimens were scanned on a nanotom micro-CT (phoenix nanotom® m, General Electric, Fairfield, CT, USA) for high resolution images.
7
Laser Scanning and Micro-CT Imaging
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Femora and pelves were collected from a single specimen of 18 species in total housed in the collections of Naturhistorisches Museum Vienna (NMW), Austria, and Museum für Naturkunde Berlin (MfN), Germany (Supplementary Table S1 ). The bones were scanned with a surface laser scanner via the software “Scantools” (Kreon “Skiron” laser scanner for MicroScribe, Solution Technologies, Inc., Oella, United States) or, if too small for surface laser scanning, using a µCT scanner (Phoenix Nanotom M, General Electric, Boston, United States) with the software VGSTUDIO MAX (Volume Graphics, Heidelberg, Germany). Minor defects of the meshed surface models were repaired using the software MeshLab version 1.3.3 (Cignoni et al., 2008 ) and Geomagic Wrap 2017 (3D Systems, Rock Hill, United States).
8
Non-Destructive Analysis of Internal Cracks in Compacts
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To study the internal micro-structures of samples and detect internal cracks in a non-destructive manner, an X-ray CT scanner using a CT-Scanner (Phoenix Nanotom ® M, General Electric, Boston, Massachusetts, USA) with a maximum voltage of 180kV, a maximum power of 20W, and an image resolution of 3072 × 2400 pixels is employed. Images were saved and then reconstructed/post-processed using Phoenix datos|x CT software (General Electric, Boston, Massachusetts, USA). Using VGStudio MAX (Volume Graphics, Charlotte, North Carolina, USA) cross-section images are constructed (Fig. 1). As depicted in Fig. 1.c, the cross-section of the compact LHS at P 1 shows a uniform microstructure with no visible cracks or breakage. In Fig. 1.d, the lateral internal cracks were observed in the compact LHS at P 2 , while the compact remained intact and no breakage was observed. As seen in Fig. 1.e, substantial material removal from the top and bottom surfaces of the compact was present and clearly visible in the compact LHS at P 3 .
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!