Anti cd8 clone 53 6.7 armenian hamster igg

To determine if the protection induced by IL-6 Tg-PbANKA/LISP2 SPZ is dependent on effector CD4⁺ or CD8⁺ T cells, cell-specific depletion experiments were performed. C57BL/6 protected mice were injected i.p. with 20 μg of anti-CD8 clone 53-6.7 Armenian hamster IgG (eBioscience, San Diego, CA) or 100 μg of rat anti mouse CD4 clone GK1.5 (ATCC TIB207) 48 h before the infection with PbNK65 WT followed by 6 injections administered every other day after the infection. The cell depletion was followed and confirmed every day by taking 10 μl of blood from the tip of the mouse tail and analyzed by flow cytometry. Blood samples were labeled with anti-CD45-AF 647 (clone 30-F11), anti-CD8a-PE (clone 5H10) from Biolegend (San Diego, CA), and anti-CD4-FITC (clone H129-19) from BD Bioscience, Mountain View, CA.

To determine if the protection induced by PbNK65 hrfΔ is dependent on effector CD4⁺ or CD8⁺ T cells, cell-specific depletion experiments were performed. C57BL/6 J Rj protected mice were injected i.p. with 20 μg of anti-CD8 clone 53–6.7 Armenian hamster IgG (eBioscience, San Diego, CA) or 100 μg of rat anti mouse CD4 clone GK1.5 (ATCC® TIB207™) 48 h before the infection with PbNK65 WT followed by 6 injections administered every other day after the infection. The cell depletion was followed and confirmed every day by taking 10 μl of blood from the tip of the mouse tail and analysed by flow cytometry.