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Enasidenib

Manufactured by Selleck Chemicals
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Sourced in United States

Enasidenib is a laboratory chemical used in research applications. It functions as an isocitrate dehydrogenase (IDH) enzyme inhibitor. Enasidenib is primarily utilized in biochemical and cell-based assays for studying IDH-related cellular processes.

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Lab products found in correlation

Ivosidenib, by Selleck Chemicals (3 mentions) Aicar, by Merck Group (2 mentions) Lc ms grade formic acid, by Thermo Fisher Scientific (2 mentions) Hplc grade acetonitrile, by Honeywell (2 mentions) Nd 646, by Selleck Chemicals (2 mentions) Ethacrynic acid ea, by Merck Group (1 mentions) Venetoclax, by MedChemExpress (1 mentions) Etoposide, by MedChemExpress (1 mentions) Midostaurin, by Selleck Chemicals (1 mentions) Gilteritinib, by Selleck Chemicals (1 mentions) Navitoclax, by MedChemExpress (1 mentions) 5 azacytidine 5 aza, by MedChemExpress (1 mentions) Mk571, by MedChemExpress (1 mentions) Azd 5991, by MedChemExpress (1 mentions) Abt 737, by MedChemExpress (1 mentions) Doxycycline, by Merck Group (1 mentions)

4 protocols using enasidenib

1

Comprehensive Chemical Reagents Sourcing

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ND-646, enasidenib, and ivosidenib were purchased from Selleck Chemicals. All other chemicals including dox, AICAR, and TOFA were purchased from Sigma-Aldrich with the exception the following LC-MS solvents: HPLC-grade acetonitrile and water were purchased from Honeywell Burdick and Jackson, and LC-MS–grade formic acid was purchased from Thermo Fisher Scientific.
Thomas D., Wu M., Nakauchi Y., Zheng M., Thompson-Peach C.A., Lim K., Landberg N., Köhnke T., Robinson N., Kaur S., Kutyna M., Stafford M., Hiwase D., Reinisch A., Peltz G, & Majeti R. (2022). Dysregulated Lipid Synthesis by Oncogenic IDH1 Mutation Is a Targetable Synthetic Lethal Vulnerability. Cancer Discovery, 13(2), 496-515.
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2

Manipulating T-B cell interactions

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Co-cultures of Tfh cells and WT B cells were established as described above. On day 1 of co-culture, various blocking antibodies were added: either isotype control; or anti-CD80 (5 mg/mL) and anti-CD86 (5 mg/mL); or anti-PD-1 (20mg/mL); or anti-CD40L (20mg/mL); or anti-ICOS (5 mg/mL); or anti-Fas (5 mg/mL); or Enasidenib (IDH2 inhibitor; Selleckchem, #S8205, 1 mM). After 4 days at 37 C, B cell survival and proliferation were assayed as described above.
Leca J., Lemonnier F., Meydan C., Foox J., El Ghamrasni S., Mboumba D.L., Duncan G.S., Fortin J., Sakamoto T., Tobin C., Hodgson K., Haight J., Smith L.K., Elia A.J., Butler D., Berger T., de Leval L., Mason C.E., Melnick A., Gaulard P, & Mak T.W. (2023). IDH2 and TET2 mutations synergize to modulate T Follicular Helper cell functional interaction with the AITL microenvironment. Cancer cell, 41(2).
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3

Pharmacological Assessment of Potential Therapeutics

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Venetoclax, AZD-4320, Navitoclax, ABT-737, AZD-5991, 5-Azacytidine (5-Aza), Etoposide and MK-571 were purchased from MedChemExpress (USA). Midostaurin, Gilteritinib, Ivosidenib and Enasidenib were acquired from Selleck Chemicals (USA). Reversan was obtained from Merck (Germany). Ethacrynic acid (EA) was purchased from Sigma-Aldrich (USA). All compounds were diluted in DMSO. Buthionine sulfoximine (BSO) was purchased from Merck (Germany) and diluted in ddH2O. For in vivo experiments, AZD-4320 was dissolved in 30% 2-Hydroxypropyl-β-cyclodextrin (HP-β-CD, Merck (Germany) solution in ddH2O (3 mM stock).
Ebner J., Schmoellerl J., Piontek M., Manhart G., Troester S., Carter B.Z., Neubauer H., Moriggl R., Szakács G., Zuber J., Köcher T., Andreeff M., Sperr W.R., Valent P, & Grebien F. (2023). ABCC1 and glutathione metabolism limit the efficacy of BCL-2 inhibitors in acute myeloid leukemia. Nature Communications, 14, 5709.
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4

Compound Procurement for Biological Assays

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ND-646, enasidenib, and ivosidenib were purchased from Selleck Chemicals (Houston, Texas, USA). All other chemicals including doxycycline, AICAR and TOFA were purchased from Sigma-Aldrich (St Louis, Missouri, USA), with the exception the following LCMS solvents: HPLC grade acetonitrile and water were purchased from Honeywell Burdick and Jackson (Morristown, New Jersey, USA), and LC/MS grade formic acid was purchased from Thermo Fisher Scientific (Rockford, Illinois, USA).
Thomas D., Wu M., Nakauchi Y., Zheng M., Thompson-Peach C.A., Lim K., Landberg N., Köhnke T., Robinson N., Kaur S., Kutyna M., Stafford M., Hiwase D., Reinisch A., Peltz G, & Majeti R. (2022). Dysregulated Lipid Synthesis by Oncogenic IDH1 Mutation Is a Targetable Synthetic Lethal Vulnerability. Cancer Discovery, 13(2), 496-515.
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