Isotope labeled internal standard samples of amino acids nsk a

Dried blood spots were used in the assay of metabolomics, which were prepared from capillary whole blood through 8-h fasting. We measured the metabolites by direct infusion mass spectrometry technology equipped with the AB Sciex 4000 QTrap system (AB Sciex, Framingham, MA, USA). High-purity water and acetonitrile were purchased from Thermo Fisher (Waltham, MA, USA), and utilized as diluting agent and mobile phase. 1-Butanol and acetyl chloride were obtained from Sigma-Aldrich (St Louis, MO, USA). Isotope-labeled internal standard samples of amino acids (NSK-A) were purchased from Cambridge Isotope Labo-ratories (Tewksbury, MA, USA), while standard samples of the leucine were purchased from Chrom Systems (Grafelfing, Germany). In brief, 8.5 mL of venous blood was drawn from each participant at 08:00 to 09.30 h in the morning after 8-h fasting. Laboratory tests were carried out at a special diagnostic laboratory. The level of lipid profiles was analyzed by an automatic biochemistry analyzer (Hitachi 7150, Tokyo, Japan). We also assayed the level of HDL-C and LDL-C by the selective solubilization method (12 (link)) (Determiner L-HDL, LDL test kit; Kyowa Medex, Tokyo, Japan).

Dried blood spots were used in the metabolomic assay, which were prepared from capillary whole blood through an 8-h fasting. We measured the metabolites by direct infusion mass spectrometry technology equipped with the AB Sciex 4000 QTrap system (AB Sciex, Framingham, MA, USA). High-purity water (7732–18–5) and acetonitrile (75-05-8) were purchased from Thermo Fisher (Waltham, MA, USA), and were utilized as diluting agent and mobile phase. 1-Butanol(71-36-3) and acetyl chloride(75-36-5) were obtained from Sigma-Aldrich (St Louis, MO, USA). Isotope-labeled internal standard samples of amino acids (NSK-A) were purchased from Cambridge Isotope Labo-ratories (Tewksbury, MA, USA), while standard samples of the Hcy(14857-77-3), Cys(52-90-4) and Met(63-68-3) were purchased from Chrom Systems (Grafelfing, Germany). In brief, 8.5 mL of venous blood was drawn from each participant at 08:00 to 09.30 h in the morning after an 8-h fasting. Laboratory tests were carried out at a specialized diagnostic laboratory. The level of lipid profiles was analyzed with an automatic biochemistry analyzer (Hitachi 7150, Tokyo, Japan). We also assayed the level of HDL-C and LDL-C by the selective solubilization.

Dried blood spots were used in the assay of metabolomics, which were prepared from capillary whole blood through 8-h fasting. The metabolites were measured by direct infusion mass spectrometry technology equipped with the AB Sciex 4000 QTrap system (AB Sciex, Framingham, MA, USA). High-purity water and acetonitrile were purchased from Thermo Fisher (Waltham, MA, USA) and utilized as diluting agent and mobile phase. 1-Butanol and acetyl chloride were obtained from Sigma-Aldrich (St Louis, MO, USA). Isotope-labeled internal standard samples of amino acids (NSK-A) were purchased from Cambridge Isotope Laboratories (Tewksbury, MA, USA), while standard samples of the leu were purchased from Chromsystems (Grafelfing, Germany). That is to say, 8.5 ml of venous blood was drawn from each participant at 0800 to 0930h after 8-h fasting. Laboratory tests were carried out at a special diagnostic laboratory. The level of lipid profiles was analyzed by an automatic biochemistry analyzer (Hitachi 7150, Tokyo, Japan). The levels of HDL-C and LDL-C were also assessed by the selective solubilization method (Determiner L-HDL, LDL test kit; Kyowa Medex, Tokyo, Japan).