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Psv40 renilla

Manufactured by Promega
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The PSV40-Renilla is a lab equipment product from Promega. It is a plasmid that contains the Renilla luciferase gene under the control of the SV40 promoter. The Renilla luciferase enzyme is commonly used as a reporter gene to monitor gene expression in various experimental systems.

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Lab products found in correlation

Dual luciferase reporter assay system, by Promega (2 mentions) Tcf reporter plasmid kit, by Merck Group (1 mentions) Dual luciferase assay kit, by Promega (1 mentions) Lipofectamine 2000 transfection reagent, by Thermo Fisher Scientific (1 mentions) Pgl3 basic, by Promega (1 mentions) Dual luciferase assay reagent, by Promega (1 mentions) Synergy h1 luminometer, by Agilent Technologies (1 mentions) Thedual glo luciferase assay system, by Promega (1 mentions)

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PSV40-Renilla plasmid (6 citations)

5 protocols using psv40 renilla

1

Wnt/β-catenin Pathway Activity Assay

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The activity of Wnt/β-catenin pathway was detected by TCF Reporter Plasmid Kit (17-285, Millipore). The mouse primary GECs were transduced with pTopFlash (TCF reporter plasmid) or pFopFlash (mutant, inactive TCF binding site) plasmids, and pSV40-Renilla (Promega, E6911) as internal reference. After transfection for 48 h, cells were centrifuged at 12000 g for 1 min to collect supernatant. The Dual-Luciferase® Reporter Assay System (E1910, Promega) was adopted to detect luciferase activity. The ratio of firefly luciferase to renilla luciferase was used as the relative activity of luciferase.
Zuo W., Yang H., Li N., Ouyang Y., Xu X, & Hong J. (2022). Helicobacter pylori infection activates Wnt/β-catenin pathway to promote the occurrence of gastritis by upregulating ASCL1 and AQP5. Cell Death Discovery, 8, 257.
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2

Determining NFκB Transcriptional Activity

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The effect of different treatments on the transcriptional activity of NFκB was determined by luciferase reporter gene assays. Transfections were performed using Lipofectamine 2000 transfection reagent according to the manufacturer's instructions (Invitrogen, Paisley, UK). Cells (1 × 104/well) were cultured in 96-well plates overnight. The luciferase reporter vectors (0.8μg/well) [pNFκB-Tal-Luc (BD Biosciences) and pGL3-Basic (Promega, Southampton, UK)] were co-transfected with 0.008 μg/well pSV40-Renilla (Promega) DNA, an internal control for normalization of the transcriptional activity of the reporter vectors. Twenty-four hours after transfection, the cells were lysed and luciferase activity was determined using Dual Luciferase Assay kit (Promega) according to the manufacturer's instructions. The luciferase activity in each well was normalized to pSV40-Renilla using Ln = L/R (Ln: normalized luciferase activity; L: luciferase activity reading; R: Renilla activity reading). The transcriptional specificity was monitored by the transcriptional activity of the pGL3-Basic. All transfections were performed in triplicate with at least duplicate independent experiments.
Liu P., Wang Z., Brown S., Kannappan V., Tawari P.E., Jiang W., Irache J.M., Tang J.Z., Britland S., Armesilla A.L., Darling J.L., Tang X, & Wang W. (2014). Liposome encapsulated Disulfiram inhibits NFκB pathway and targets breast cancer stem cells in vitro and in vivo. Oncotarget, 5(17), 7471-7485.
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3

Luciferase Reporter Assay for Transcriptional Activity

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The luciferase reporter vectors were co-transfected with pSV40-Renilla (Promega) vector, internal control for normalization of the transcriptional activity of the reporter vectors. Forty-eight hours after transfection, the cells were cultured with the indicated treatment conditions, and then luciferase activity was subsequently determined using Dual-luciferase assay reagents (Promega) according to the manufacturer’s protocol. Each luciferase activity was normalized to internal control, pSV40-Renilla.
Jung J., Zhang Y., Celiku O., Zhang W., Song H., Williams B.J., Giles A.J., Rich J.N., Abounader R., Gilbert M.R, & Park D.M. (2019). Mitochondrial NIX Promotes Tumor Survival in the Hypoxic Niche of Glioblastoma. Cancer research, 79(20), 5218-5232.
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4

Measuring FOXO Transcriptional Activity

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A reporter construct, pGL3-FHRE-Luc was originally from M. Greenberg (Addgene Plasmid 1789), which expresses the firefly luciferase driven by a promoter containing three copies of forkhead responsive elements, was employed to measure FOXO transcription activity [80] (link). A control reporter construct, pSV40-Renilla (Promega), which provides constitutive expression of Renilla luciferase, was used as an internal control. Cells were plated in 24-well plates at a density of 1×105 cells per well, and after 24 h, cells were transfected with expression plasmids of FOXOs, pSV40-Renilla, pGL3-FHRE-luciferase, or TDP-43. At 48 h, luciferase activities were measured by using the Dual-Luciferase Reporter Assay System (Promega) on a Synergy H1 luminometer (Bio-Tek). The experimental firefly luciferase activity was normalized to the control Renilla luciferase activity to reflect the FOXO activities.
Zhang T., Baldie G., Periz G, & Wang J. (2014). RNA-Processing Protein TDP-43 Regulates FOXO-Dependent Protein Quality Control in Stress Response. PLoS Genetics, 10(10), e1004693.
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5

Luciferase Assay for Odorant Receptor-I7 Activation

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The
Dual-Glo Luciferase Assay System (Promega) was used for the
luciferase assay as previously described.41 (link) Briefly, a plasmid encoding Rho-tagged rat OR-I7 (5 ng/well) was
transfected into the Hana3A cell line in 96-well plate format along
with plasmids encoding the human receptor trafficking protein, RTP1S40 (link) (5 ng/well), pSV40-Renilla (5
ng/well; Promega), CRE-luciferase (10 ng/well; Stratagene), and type
3 muscarinic acetylcholine receptor (M3-R)39 (2.5 ng/well). Then, 18 to 24 h following transfection, cells were
treated with compound 2 for 4 h at 37 °C, as described.39 Luminescence was measured using a Polarstar
Optima plate reader (BMG). Luciferase measurements were normalized
to Renilla luciferase measurements to control for
transfection efficiency and cell viability. Fold change values were
calculated by the formula (F1–F0)/F0, where F1 is the normalized luminescence response to
the odorant and F0 is the normalized luminescence
when no odorant was applied. Data were analyzed and the EC50 for 2 (≈10 μM) was estimated using Prism
5.0 and Microsoft Excel. Estimating the EC50s for the other
four odorants under the conditions of this assay was not possible
because they underwent significant evaporation. For this reason, we
used the GloSensor and calcium imaging assays described above to monitor
OR-I7 activation in real time.
Li Y., Peterlin Z., Ho J., Yarnitzky T., Liu M.T., Fichman M., Niv M.Y., Matsunami H., Firestein S, & Ryan K. (2014). Aldehyde Recognition and Discrimination by Mammalian Odorant Receptors via Functional Group-Specific Hydration Chemistry. ACS Chemical Biology, 9(11), 2563-2571.
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