S raclopride tartrate salt

Cocaine, D₁R antagonist [R(+)-SCH-23390 hydrochloride] and D₂R antagonist [S(-)-raclopride(+)-tartrate salt] were purchased from the Sigma Chemical Company (St. Louis, MO, USA). All chemicals were dissolved immediately in physiological saline before use. The CART and GAPDH primers, M-MLV reverse transcriptase and AccuPower PCR PreMix were purchased from Bioneer Co. (Seoul, Korea). The 2X SYBR Green Master Mix was purchased from Applied Biosystems (Carlsbad, CA, USA). The anti-CART and anti-GAPDH antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The anti-phospho[Ser-133] CREB, anti-CREB, and anti-PKA antibodies were purchased from Abcam (Cambridge, MA, USA). The Vectastain ABC reagent was purchased from Vector Laboratories Co. (Burlingame, CA, USA). The Cyclic AMP XP™ Assay Kit was purchased from Cell Signaling Technology Inc. (Danvers, MA, USA). Protein G Sepharose 4 Fast Flow was purchased from GE Healthcare Bio-Sciences AB (Uppsala, Sweden). The PKA Assay kit was purchased from Millipore Co. (Bedford, MA, USA). The [γ-³²P] ATP was purchased from Perkin Elmer, Inc. (Covina, CA, USA). All other materials were of the highest grade available and were obtained from normal commercial sources.