1 3 dimethylaminopropyl 3 ethylcarbodiimide hydrochloride edc hcl

1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC-HCl) was purchased from Tokyo Chemical Industry (Tokyo, Japan). Bis(3-aminopropyl) terminated poly(dimethylsiloxane) (H2N-PDMS-NH2, Mn = 5000–7000) were purchased from Gelest All other chemicals were purchased from Sigma-Aldrich unless stated otherwise.

Alginic acid sodium salt from brown algae (medium viscosity), dopamine hydrochloride, N-hydroxysuccinimide (NHS), 2-(N-morpholino)ethanesulfonic acid (MES) solution (1 M), Dulbecco’s phosphate buffered saline (DPBS) with calcium chloride and magnesium chloride, Trizma base, Trizma hydrochloride, and N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) (HEPES) buffer solution (1 M) were purchased from Sigma Aldrich (St. Louis, MO, USA). 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC-HCl) was purchased from Tokyo Chemical Industry (Tokyo, Japan). Phosphate buffered saline (PBS, 10X, pH 7.2) was purchased from WELGENE (Gyeongsan, Korea). Anhydrous ethyl alcohol was purchased from Daejung (Siheung, Korea). Fetal bovine serum (FBS, qualified), penicillin-streptomycin and Dulbecco modified eagle medium (DMEM) were purchased from Gibco (Waltham, MA, USA). LIVE/DEAD viability/cytotoxicity kit for mammalian cells was purchased from Invitrogen (Waltham, MA, USA). SYLGARD 184 silicone elastomer base and SYLGARD 184 silicone elastomer curing agent were purchased from Dow (Midland, MI, USA). SpectraPor 1 Dialysis Membrane (Standard RC Tubing, molecular weight cut off (MWCO) = 6–8 kDa) was purchased from Spectrum (Rancho Dominguez, CA, USA).

Stearoyl chloride and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC-HCl) were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Triethylamine, tetrahydrofuran (THF) super dehydrated with stabilizer (super dehydrated), NHS, N, N-dimethylformamide (DMF), hydrogen chloride (HCl), and BSA were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Roswell Park Memorial Institute (RPMI) medium was obtained from Nissui Pharmaceutical Co., Ltd. (Tokyo, Japan), and fetal bovine serum (FBS) was obtained from Equitech-Bio, Inc. (Kerrville, TX, USA). Opti-modified Eagle’s medium (Opti-MEM) was purchased from Thermo Fisher Scientific K.K. (Tokyo, Japan). All other chemicals were of the highest grade available and used without further purification.