Wild type laboratory-bred AB strain zebrafish were held in breeding groups of 30 females and 30 males at the Department of Aquaculture, Szent István University, Hungary, in a Tecniplast ZebTEC recirculation system (Tecniplast S.p.a., Buguggiate, Italy) at 25.5 °C ± 0.5 °C, pH 7.0 ± 0.2, conductivity 550 ± 50 µS (system water), and a light:dark period of 14 h:10 h. Fish were fed twice a day with dry granulate food (Zebrafeed 400–600 µm, Sparos Lda., Olhão, Portugal) supplemented with freshly hatched live Artemia salina once a day. Fish were placed in breeding tanks (Tecniplast S.p.a.) late in the afternoon the day before the experiment and allowed to spawn by removing the dividing walls the next morning. Spawning of individual pairs was delayed through time to allow a continuous supply of one-cell embryos.
Zebtec recirculation system
Manufactured by Tecniplast
Sourced in Italy
The Tecniplast ZebTEC recirculation system is a piece of lab equipment designed for the housing and maintenance of aquatic organisms, such as zebrafish. The system provides a controlled and monitored environment for the animals, including water filtration, temperature regulation, and other essential functions to support their well-being. The core function of the ZebTEC system is to maintain a suitable habitat for the animals under study, without making claims about its intended use or applications.
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Lab products found in correlation
6 protocols using zebtec recirculation system
1
Zebrafish Breeding and Embryo Production
2
Zebrafish Breeding and Embryo Collection
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Laboratory-bred AB strain zebrafish were held in breeding groups of 30 females and 30 males at the Institute of Aquaculture and Environmental Safety, Hungarian University of Agriculture and Life Sciences, Hungary, in a Tecniplast ZebTEC recirculation system (Tecniplast S.p.A., Buguggiate, Italy) at 25.5 ± 0.5 °C, pH 7.0 ± 0.2, conductivity 550 ± 50 µS (system water), and light:dark period of 14 h:10 h. Fish were fed twice a day with dry granule food (Zebrafeed 400–600 µm, Sparos Lda., Olhão, Portugal) supplemented with freshly hatched live Artemia salina twice a week. Fish were placed in breeding tanks (Tecniplast S.p.A.) late in the afternoon the day before the experiment and allowed to spawn by removing the dividing walls the next morning. Spawning of individual pairs was delayed through time to allow a continuous supply of 1-cell embryos.
3
Zebrafish Breeding and Embryo Production
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Laboratory-bred wild type AB strain zebrafish were held in breeding groups of 30 females and 30 males at the Department of Aquaculture, Szent István University, Hungary, in a Tecniplast ZebTEC recirculation system (Tecniplast S.p.A., Italy) at 25.5 °C ± 0.5 °C, pH 7.0 ± 0.2, conductivity 550 ± 50 µS (system water) and a light: dark period of 14 h:10 h. The fish were fed twice a day with dry granulate food (Zebrafeed 400–600 µm, Sparos Lda., Portugal) supplemented with freshly hatched live Artemia salina once a day. The fish were placed in breeding tanks (Tecniplast S.p.a.) late in the afternoon the day before the experiment and allowed to spawn by removing the dividing walls the following morning. Spawning of individual pairs was delayed to allow for a continuous supply of 1-cell embryos.
4
Zebrafish Breeding and Embryo Collection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Laboratory-bred AB strain zebrafish were held in breeding groups of 30 females and 30 males at the Department of Aquaculture, Szent István University, Hungary, in a Tecniplast ZebTEC recirculation system (Tecniplast S.p.A., Italy) at 25.5°C ± 0.5°C, pH 7.0±0.2, conductivity 550±50 µS (system water) and light:dark period of 14 h:10 h. Fish were fed twice a day with dry granulate food (Zebrafeed 400-600 µm, Sparos Lda., Portugal) supplemented with freshly hatched live Artemia salina twice a week. Fish were placed in breeding tanks (Tecniplast S.p.a.) late in the afternoon the day before the experiment and allowed to spawn by removing the dividing walls next morning. Spawning of individual pairs was delayed through time to allow a continuous supply of 1-cell embryos.
5
Zebrafish Breeding Protocol for Continuous Embryo Supply
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Laboratory-bred wild type AB strain zebrafish were held in breeding groups of 30 females and 30 males at the Department of Aquaculture, Szent István University, Hungary, in a Tecniplast ZebTEC recirculation system (Tecniplast S.p.a., Buguggiate VA, Italy) at 25.5 °C ± 0.5 °C, pH 7.0 ± 0.2, conductivity 550 ± 50 µS (system water) and light/dark period of 14 h:10 h. Fish were fed twice a day with dry granulate food (Zebrafeed 400–600 µm, Sparos Lda., Olhão, Portugal) supplemented with freshly hatched live Artemia salina twice a week. Fish were placed in breeding tanks (Tecniplast S.p.a.) late in the afternoon the day before the experiment and allowed to spawn by removing the dividing walls the next morning. Spawning of individual pairs was delayed through time to allow a continuous supply of one-cell embryos.
6
Breeding and Embryo Incubation of Transgenic Zebrafish
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Laboratory-bred Tg(vtg1:mCherry) zebrafish strain was held in breeding groups of 30 females and 30 males at the Department of Aquaculture (Szent István University, Hungary) in a Tecniplast ZebTEC recirculation system (Tecniplast S.p.a., Italy) at 25.5 ± 0.5 °C (system water: pH 7.0 ± 0.2, conductivity 550 ± 50 µS) and on a 14h:10 h light:dark cycle. The fish were fed twice a day with dry granulate food (Zebrafeed 400-600 µm, Sparos Lda., Portugal) supplemented with freshly hatched live Artemia salina once a day. The fish were placed in breeding tanks (Tecniplast S.p.a.) late in the afternoon before the day of the experiment and allowed to spawn by removing the dividing walls next morning. The collected eggs were incubated in system water with methylene blue (2 mL 0.1% methylene blue in 1 L system water) (25 ± 2 °C) in Petri dishes (diameter: 10 cm). After 24 h, coagulated and/or nonfertilized eggs were assorted, and a part of the embryos were disinfected with bleaching method to keep the experiment sterile.
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