NanoLuc® Binary Technology (NanoBiT) assays were performed with 2×105 cells/ml HEK293T cells transiently transfected with 1 μg/ml of the N-LgBiT-RING1B plasmid (full length RING1B or RING1B R98A cloned into pBiT1.1-N[TK LgBiT vector, Promega) and 0.25 μg/ml of the SmBiT-H3.3 plasmid (CMV/H3.3-SmBiT vector, Promega), and 7:1 FuGENE® HD Transfection Reagent-to-DNA ratio (Promega) in Opti-MEM™ (Gibco). After 24 h, cells were trypsinized and transferred to white 96-well tissue culture plates (30,000 cells in 100 μl per well) and incubated with compounds or 0.25% DMSO. After another 24 h, 25 μl of Nano-Glo® Live Cell Assay System (Promega) were added to each well and NanoLuc® Luminescence was detected in a PHERAstar BMG microplate reader.
Fugene hd transfection reagent to dna ratio
Manufactured by Promega
FuGENE® HD Transfection Reagent-to-DNA ratio is a reagent used for the transfer of genetic material into eukaryotic cells. The recommended ratio of reagent-to-DNA is provided by the manufacturer to ensure optimal transfection efficiency.
Automatically generated - may contain errors
2 protocols using fugene hd transfection reagent to dna ratio
1
NanoLuc Binary Technology (NanoBiT) Assay
2
NanoLuc Binary Technology (NanoBiT) Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
NanoLuc® Binary Technology (NanoBiT) assays were performed with 2×105 cells/ml HEK293T cells transiently transfected with 1 μg/ml of the N-LgBiT-RING1B plasmid (full length RING1B or RING1B R98A cloned into pBiT1.1-N[TK LgBiT vector, Promega) and 0.25 μg/ml of the SmBiT-H3.3 plasmid (CMV/H3.3-SmBiT vector, Promega), and 7:1 FuGENE® HD Transfection Reagent-to-DNA ratio (Promega) in Opti-MEM™ (Gibco). After 24 h, cells were trypsinized and transferred to white 96-well tissue culture plates (30,000 cells in 100 μl per well) and incubated with compounds or 0.25% DMSO. After another 24 h, 25 μl of Nano-Glo® Live Cell Assay System (Promega) were added to each well and NanoLuc® Luminescence was detected in a PHERAstar BMG microplate reader.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!