Expression of human NSCLC stem cell marker CD133 and CD44 was analyzed by flow cytometric study in A549 cells and spheroids by using anti-CD133-FITC and anti-CD44-APC antibodies (BD Biosciences). CD133+ and CD44+ CSCs were flow-cytometrically gated from spheroids on the basis of the cell surface phenotype. Mean fluorescence intensities of Oct-4-PerCP-Cy5.5, Nanog-PE, Sox-2- Alexa Fluor-647, Mrp1-FITC, Aldh1-FITC (BD Biosciences) were quantified22 (link). Mean fluorescence intensities of Akt, p-Akt, Cxcr-4, Mdr1, Integrin-α2, Integrin-α5, Integrin-β1, p-Fak (Santa Cruz Biotechnology, Inc.) were determined with respective primary antibodies conjugated with PE as previously described13 (link).
Sox 2 alexa fluor 647
Manufactured by BD
Sourced in Germany
Sox-2-Alexa Fluor-647 is a fluorescently labeled antibody that targets the Sox-2 transcription factor. Alexa Fluor-647 is the fluorescent dye conjugated to the antibody, providing a means for detection and visualization of Sox-2 in various experimental applications.
Automatically generated - may contain errors
Lab products found in correlation
Nanog pe, by BD (2 mentions)
P fak, by Santa Cruz Biotechnology (1 mentions)
P akt, by Santa Cruz Biotechnology (1 mentions)
Mrp1 fitc, by BD (1 mentions)
Aldh1 fitc, by BD (1 mentions)
Anti cd44 apc, by BD (1 mentions)
Integrin α2, by Santa Cruz Biotechnology (1 mentions)
Integrin α5, by Santa Cruz Biotechnology (1 mentions)
Integrin β1, by Santa Cruz Biotechnology (1 mentions)
Cxcr4, by Santa Cruz Biotechnology (1 mentions)
Cd33 pe, by Miltenyi Biotec (1 mentions)
Ssea3 pe, by BD (1 mentions)
Lsrii flow cytometer, by BD (1 mentions)
Facsdiva software, by BD (1 mentions)
Cd45 apc, by Miltenyi Biotec (1 mentions)
Cytofix cytoperm kit, by BD (1 mentions)
Cd34 pe, by Miltenyi Biotec (1 mentions)
2 protocols using sox 2 alexa fluor 647
1
Flow Cytometric Analysis of NSCLC Stem Cell Markers
2
Multiparametric Flow Cytometry Analysis
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Single-cell suspensions were stained using combinations of the following antibodies: CD13-FITC, CD33-PE, CD34-PE, CD45-APC (Miltenyi Biotech, Bergisch Gladbach, Germany, http://www.miltenyibiotec.com ) for hematopoietic phenotyping; SSEA3-PE and TRA-1–60-Alexa Fluor 647 (BD Pharmingen) for live extracellular pluripotent phenotyping; and OCT4-Alexa Fluor 488, SOX2-Alexa Fluor 647, and NANOG-PE (BD Biosciences) for intracellular pluripotent phenotyping of cells fixed and permeabilized using the BD Cytofix/Cytoperm kit. Flow cytometry was performed using the LSRII Flow Cytometer with FACSDiva software (Becton-Dickinson, Franklin Lakes, NJ, http://www.bd.com ) and analyzed by FlowJo software (Tree Star, Inc., Ashland, OR, http://www.treestar.com ).
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