Cd4 cy55pe

Manufactured by Thermo Fisher Scientific

The CD4-Cy55PE is a fluorescently labeled antibody for the detection and quantification of CD4-positive cells by flow cytometry. It is a comprehensive and reliable tool for immunophenotyping and monitoring of CD4+ T cells.

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Lab products found in correlation

Ifn γ efluor450, by Thermo Fisher Scientific (1 mentions) Cd8 bv711, by BioLegend (1 mentions) Ctla4 apc, by BD (1 mentions) Cd3 apc h7, by BD (1 mentions) Aqua amine reactive dye, by Thermo Fisher Scientific (1 mentions) Cd27 alexa fluor700, by BD (1 mentions) Mip1β pe, by BD (1 mentions) Ctla 4 apc, by Beckman Coulter (1 mentions) Cd127 cy5pe, by BioLegend (1 mentions) Ccr7 bv605, by Thermo Fisher Scientific (1 mentions) Cd45ra cy7pe, by Beckman Coulter (1 mentions) Btla pe, by BioLegend (1 mentions) Pd 1 bv421, by BioLegend (1 mentions) Cd27 qd605, by Thermo Fisher Scientific (1 mentions) Cd3 h7apc, by BD (1 mentions) Ccr7 alexa700, by Beckman Coulter (1 mentions) Ccr6 alexa647, by Thermo Fisher Scientific (1 mentions) Il 17a cy5.5percp, by Thermo Fisher Scientific (1 mentions) Cd154 cy5pe, by Thermo Fisher Scientific (1 mentions) Cd160 pe, by BioLegend (1 mentions)

2 protocols using cd4 cy55pe

Multiparameter Flow Cytometry Panel

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The following directly conjugated antibodies were used: CD3-APCH7, CD27-Alexa Fluor700, MIP1β-PE, CTLA4-APC, TNFα-Cy7PE, IL2-Cy55PCP (BD Biosciences); CD4-Cy55PE, Streptavidin (SA)-quantum dot (QD) 655, Aqua amine reactive dye (Invitrogen); CD45RO-TRPE, 2B4-Cy5PE (Beckman Coulter); IFNγ-eFluor450 (eBioscience); Biotinylated PD-1 (clone BAF1086; R&D Systems); CD8-BV711 (Biolegend). The following antibodies were conjugated in our laboratory: CD8-QD800 and CD57-QD565.

Saharia K.K., Petrovas C., Ferrando-Martinez S., Leal M., Luque R., Ive P., Luetkemeyer A., Havlir D, & Koup R.A. (2016). Tuberculosis Therapy Modifies the Cytokine Profile, Maturation State, and Expression of Inhibitory Molecules on Mycobacterium tuberculosis-Specific CD4+ T-Cells. PLoS ONE, 11(7), e0158262.

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Comprehensive Immune Phenotyping by Flow Cytometry

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Flow cytometry was performed using the following directly conjugated antibodies:
(1) BD Biosciences: CD3-H7APC (SK7), CD45RA-Cy7PE (L48), CTLA-4-APC (BNI3),
IFN-g-FITC (B27), CCR7-Alexa700 (150503), CCR5-FITC (2D7/CCR5), CCR4-PE (1G1) and
IL-2-PE (MQ1-17H12), Ki67-FITC (B56); (2) Beckman Coulter: CD27-Alexa680
(IA4CD27), CD127-Cy5PE (R34.34), CD160-PE (BY55), BTLA-PE (J168-540); (3)
BioLegend: PD-1-BV421 (EH12.2H7), 2B4-FITC (CD244, C1.7), IL-17a-Cy5.5PerCP
(BL168), CCR6-Alexa647 (or PE, TG7/CCR6), CD27 Alexa647 (O323), CCR7 BV605
(G043H7), CXCR5 BV421 (J252D4), and CD154-Cy5PE (24–31); (4) Invitrogen:
CD4-Cy5.5PE (S3.5), CD27-QD605 (CLB-27/1), CD8-QD800 (3B5). A biotinylated
anti-PD-1 antibody was obtained from R&D (BAF 1086) and streptavidin-Qdot
655 was obtained from Molecular Probes. Quantum dots and Aqua amine viability dye
were obtained from Invitrogen. CD27-Alexa 594, TNF-a Alexa 594, HLA-DR BV650, and
CD38-Alexa 680 were conjugated in-house.

Paris R.M., Petrovas C., Ferrando-Martinez S., Moysi E., Boswell K.L., Archer E., Yamamoto T., Ambrozak D., Casazza J.P., Haubrich R., Connors M., Ake J., Kim J.H, & Koup R.A. (2015). Selective Loss of Early Differentiated, Highly Functional PD1high CD4 T Cells with HIV Progression. PLoS ONE, 10(12), e0144767.

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