Moticam 2500 5.0m pixel live resolution camera

THP-1 macrophages were incubated with TRLs at the same conditions as described in Section 2.10. After incubation, macrophages were washed with PBS and treated with 1 mL of isopropanol 60% for 2 min. Once isopropanol was removed, 1 mL of Oil Red O (0.2% (m/v) in 40% isopropanol/water (v/v)) was added and cells were incubated at room temperature for 10 min. Oil Red O was removed, and cells were washed with PBS. Finally, 2 mL of glycerol 30% in water (v/v) was added for preservation for 3–4 days. An inverted microscope Motic AE21 Series (Barcelona, Spain) coupled to a Moticam 2500 5.0M Pixel Live Resolution camera (Motic, Barcelona, Spain) was used for microphotography.

BV2 cells were seeded at 5 × 10⁵ cells/mL and treated with TRL for 24 h at 37 °C. After incubation, cells were washed with PBS and treated with isopropanol 60% for 2 min. Isopropanol was removed and Oil Red O (0.2% (m/v) in 40% isopropanol/water (v/v)) was added and incubated for 10 min. Cells were washed with PBS and glycerol (30% in water, v/v) was added for preservation. A Motic AE21 Series (Motic, Barcelona, Spain) microscope coupled to a Moticam 2500 5.0 M Pixel Live Resolution camera (Motic, Barcelona, Spain) was used for microphotography.