Anti cd4 bb700

Manufactured by BD

Sourced in United States

Anti-CD4-BB700 is a flow cytometry reagent that binds to the CD4 antigen on human cells. It is designed for the identification and analysis of CD4-positive cells in biological samples using flow cytometry.

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Lab products found in correlation

Anti cd3 fitc, by BD (2 mentions) Anti cd8 bv510, by BD (1 mentions) Facscanto 2, by BD (1 mentions) Anti cd3 apc cy7, by BD (1 mentions) Anti tnf α pe, by BD (1 mentions) Anti il 2 bv605, by BD (1 mentions) Anti ifnγ alexa fluor 647, by BD (1 mentions) Brefeldin a, by BD (1 mentions) Cytoflex s, by Beckman Coulter (1 mentions) Lsrfortessa x 20 flow cytometry, by BD (1 mentions) Anti gata3 bv421, by BD (1 mentions) Anti cd4 fitc, by BD (1 mentions)

Close spelling variants of this product

Anti-mouse CD4-BB700 CD4-BB700 BB700 Anti-CD4

3 protocols using anti cd4 bb700

Immune Cell Phenotyping by Flow Cytometry

Cited 1 time

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Two milliliters of peripheral blood were collected from participants and placed in an ethylene diamine tetraacetic acid (EDTA) anticoagulant tube for routine blood tests during recruitment. Another 100 μL of peripheral blood was taken to be labeled with antibodies. After antibody labeling, the red blood cells were lysed and washed with phosphate-buffered saline. The antibody labels were then detected. The labeled antibodies were described in previous literature [25 (link)] as follows: anti-CD3-allophycocyanin (APC)-cyanine (Cy) 7 (clone SK7), anti-CD4-BB700 (clone SK3), anti-CD8-BV510 (clone SK1), anti-CD25-phycoerythrin (PE) (clone M-A251), anti-CD127-BV421 (clone HIL-7R-M21), anti-CD62L-APC (clone DREG-56), anti-CD45RO-BB515 (clone UCHL1), and anti-HLA-DR-PE-Cy7 (clone G46-6). One hundred microliters of peripheral blood were also collected from the homotypic controls for antibody labeling. Homotypic controls were tested using anti-CD3-APC-Cy7 (clone SK7), anti-CD4-BB700 (clone SK3), anti-CD8-BV510 (clone SK1), immunoglobulin 1 (IgG1) k-PE (clone MOPC-21), IgG1 k-BV421 (clone X40), IgG1 k-APC (clone MOPC-21), IgG2a k-BB515 (clone G155-178), and IgG2a k-PE-Cy7 (clone G155-178) (BD Biosciences).
Flow cytometry BD FACS Canto™ II was applied for data collection and FlowJo V10 software (Tree Star, Inc., Ashland, OR, USA) was utilized for data analysis.

Zhang L., Nie X., Luo Z., Wei B, & Teng G. (2021). The Role of Human Leukocyte Antigen-DR in Regulatory T Cells in Patients with Virus-Induced Acute Exacerbation of Chronic Obstructive Pulmonary Disease. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 27, e928051-1-e928051-13.

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Cytokine Production Analysis of Mouse Splenocytes

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ICS was processed according to our previous method [87 (link)]. Briefly, 1×10⁶ freshly isolated mouse splenocytes were stimulated with HSV-1 peptides (Genscript, Nanjing, China, listed in S3 Table at a final concentration of 2 mg/mL for 2 h at 37°C. The splenocytes were then incubated with brefeldin A (BD Pharmingen) for 16 h at 37°C. After incubation, the cells were collected and stained with anti-CD3-FITC, anti-CD4-BB700, and anti-CD8-PE-Cy7 monoclonal antibodies (BD Biosciences) for 1 h. Then the resuspended cells were permeabilized in FACS Perm/wash buffer for 20 min before staining with anti-IFN-γ-Alexa Fluor 647, anti-TNF-α-PE, and anti-IL-2-BV605 (BD Pharmingen). Samples were tested using the flow cytometer (CytoFLEX S, Beckman, America) instrument with CytExpert software (version 2.4).

Zhao J., Chen J., Wang C., Liu Y., Li M., Li Y., Li R., Han Z., Wang J., Chen L., Shu Y., Cheng G, & Sun C. (2022). Kynurenine-3-monooxygenase (KMO) broadly inhibits viral infections via triggering NMDAR/Ca2+ influx and CaMKII/ IRF3-mediated IFN-β production. PLoS Pathogens, 18(3), e1010366.

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Immune Cell Profiling in Allergic Dermatitis

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Immune cells were isolated from the spleen and lymph node of OVA-induced AD mice. The cells were stained with anti-CD4-FITC and anti-CD8-APC antibodies (BD Biosciences, San Jose, CA, USA). The differentiated Th2 cells were stained with anti-CD3-FITC, anti-CD4-BB700, and anti-GATA3-BV421 (BD Biosciences). After staining, the populations of CD4+ T cells, CD8+ T cells, and Th2 cells were analyzed using a LSRFortessa™ X-20 flow cytometry (BD Biosciences) and FlowJo v. 10 software (FlowJo, Ashland, OR, USA).

Park S.H., Song H.K., Ji K.Y., Jung D.H., Jang S., Kim T, & Kim H.K. (2023). Topical Administration of Gardenia jasminoides Extract Regulates Th2 Immunity in OVA-Induced Mice. Cells, 12(6), 941.

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