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13xlexaop2 ivs gcamp6s p10

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The 13XLexAop2-IVS-GCaMP6s-p10 is a genetically encoded calcium indicator. It is designed to report neuronal activity by producing fluorescent signals in response to changes in intracellular calcium concentration.

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Lab products found in correlation

Uas trpa1, by Bloomington Drosophila Stock Center (2 mentions) Th gal4, by Bloomington Drosophila Stock Center (2 mentions) Alrm lexa gad, by Bloomington Drosophila Stock Center (2 mentions) Uas wtrw gfp, by Bloomington Drosophila Stock Center (2 mentions) Lexaop r geco1, by Bloomington Drosophila Stock Center (2 mentions) Tdc2 gal4, by Bloomington Drosophila Stock Center (2 mentions) 20xuas ivs gcamp6s, by Bloomington Drosophila Stock Center (2 mentions) Uas kir2.1 egfp, by Bloomington Drosophila Stock Center (2 mentions) Uas oct tyrrrnai, by Bloomington Drosophila Stock Center (2 mentions) Uas shidn, by Bloomington Drosophila Stock Center (2 mentions) Uas cschrimson, by Bloomington Drosophila Stock Center (2 mentions) Adorkg03964ex, by Bloomington Drosophila Stock Center (2 mentions) Tdc2 lexa, by Bloomington Drosophila Stock Center (2 mentions) Th lexa, by Bloomington Drosophila Stock Center (2 mentions) Tsh gal8033, by Bloomington Drosophila Stock Center (2 mentions) Uas dtrpa1, by Bloomington Drosophila Stock Center (1 mentions) Gr66a gal4, by Bloomington Drosophila Stock Center (1 mentions) Uas rpr, by Bloomington Drosophila Stock Center (1 mentions) Uas campari, by Bloomington Drosophila Stock Center (1 mentions)

3 protocols using 13xlexaop2 ivs gcamp6s p10

1

Drosophila Neuronal Characterization Protocol

Cited 1 time
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Flies were cultured in standard cornmeal food at 25°C in 12h/12h light/dark cycles. Fly stocks include: w1118, alrm-Gal426 (link), alrm-LexA::GAD, UAS-wtrw-gfp, LexAop-R-GECO1 (Bloomington, 52224), tdc2-Gal4 (Bloomington, 9313), 20XUAS-IVS-GCaMP6s (Bloomington, 42746), 13XLexAop2-IVS-GCaMP6s-p10 (Bloomington, 44274), UAS-Kir2.1-EGFP (Bloomington, 6595), UAS-Oct-TyrRRNAi (Bloomington, 28332), th-Gal4 (Bloomington, 8848), UAS-wtrwVDRC (VDRC, 107423), Oct-TyrRhono (Kyoto, 109038), UAS-shiDN (Bloomington, 5822), UAS-TrpA1 (Bloomington, 26263), UAS-NpHR (Kyoto, 117008), UAS-CsChrimson (Bloomington, 55135), AdoRKG03964ex (Bloomington, 30868), tdc2-LexA::p6527 (link), UAS-wtrw2.2328 (link), wtrwex29 (link), tβhnM1830 , tdc2RO5431 , th-LexA::p6532 , tsh-Gal8033 .
Ma Z., Stork T., Bergles D.E, & Freeman M.R. (2016). Neuromodulators signal through astrocytes to alter neural circuit activity and behavior. Nature, 539(7629), 428-432.
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2

Genetic Tools for Neuromodulation in Drosophila

Cited 2 times
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Wild type (OrgR) crossed to UAS-dTrpA1 (Bloomington #26263) served as control in Figs 1, 2, 3 and 5. Hugin-Gal4 (HugS3-Gal4 (ref. 13 (link)), Bloomington# 58769), GR66a-Gal4 (second Chr., gift from K.Scott31 (link)(formerly described as GR66C1)), GR66a-Gal4 (third Chr., Bloomington# 57670 used in Fig. 7b), Hugin-lexA (Hug1.2-lexA attp40 (ref. 32 (link))), HugPC-Gal4 (see generation of this Gal4-line below), UAS-eNpHR-YFP (Bloomington# 41753, referred here as UAS-YFP in Supplementary Fig. 3, since this homozygous line together with HugPC-Gal4 was used as fluorescent marker only), UAS-CaMPARI (Bloomington #58761), UAS-rpr;;UAS-hid (UAS-rpr (Bloomington# 5823) crossed homozygous to UAS-hid33 (link)), UAS-shibireTS34 (link), lexAop-CD4::spGFP11 and UAS-CD4::spGFP1-10 were gifts from K. Scott19 (link), 13x LexAop2-IVS-GCamP6s-p10 (Bloomington #44274).
Hückesfeld S., Peters M, & Pankratz M.J. (2016). Central relay of bitter taste to the protocerebrum by peptidergic interneurons in the Drosophila brain. Nature Communications, 7, 12796.
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3

Drosophila Neuronal Characterization Protocol

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Flies were cultured in standard cornmeal food at 25°C in 12h/12h light/dark cycles. Fly stocks include: w1118, alrm-Gal426 (link), alrm-LexA::GAD, UAS-wtrw-gfp, LexAop-R-GECO1 (Bloomington, 52224), tdc2-Gal4 (Bloomington, 9313), 20XUAS-IVS-GCaMP6s (Bloomington, 42746), 13XLexAop2-IVS-GCaMP6s-p10 (Bloomington, 44274), UAS-Kir2.1-EGFP (Bloomington, 6595), UAS-Oct-TyrRRNAi (Bloomington, 28332), th-Gal4 (Bloomington, 8848), UAS-wtrwVDRC (VDRC, 107423), Oct-TyrRhono (Kyoto, 109038), UAS-shiDN (Bloomington, 5822), UAS-TrpA1 (Bloomington, 26263), UAS-NpHR (Kyoto, 117008), UAS-CsChrimson (Bloomington, 55135), AdoRKG03964ex (Bloomington, 30868), tdc2-LexA::p6527 (link), UAS-wtrw2.2328 (link), wtrwex29 (link), tβhnM1830 , tdc2RO5431 , th-LexA::p6532 , tsh-Gal8033 .
Ma Z., Stork T., Bergles D.E, & Freeman M.R. (2016). Neuromodulators signal through astrocytes to alter neural circuit activity and behavior. Nature, 539(7629), 428-432.
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