It was performed as described earlier [22 (link), 25 (link)]. Briefly, cells plated at 50–60% confluence in 12-well plates were co-transfected with 0.25 μg of tk-PPREx3-Luc, a PPRE-dependent luciferase reporter construct, and 12.5 ng of pRL-TK (a plasmid encoding Renilla luciferase, used as transfection efficiency control; Promega) using Lipofectamine Plus (Invitrogen Life Technologies). After 24 h of transfection, cells were stimulated with different doses of gemfibrozil for 2 h. Firefly and Renilla luciferase activities were analyzed in cell extracts using the Dual Luciferase kit (Promega) in a GloMax 96 microplate luminometer (Promega) and corresponding GloMax 96 microplate luminometer software (Promega) per the manufacturer’s specifications.
Glomax 96 microplate luminometer software
Manufactured by Promega
The GloMax 96 microplate luminometer software is a tool used for measuring luminescence in microplates. It provides a platform for data collection, analysis, and reporting of luminescence-based assays.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using glomax 96 microplate luminometer software
1
Transcriptional Regulation of PPAR Targets
2
Measuring TNF-α Induced NF-κB Activation
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TZM-BL cells were transfected with siRNA targeting MAT2A (siMAT2A: GTGGCAAAATCCCTTGTTA) by using Lipofectamine RNAiMAX (ThermoFisher) according to the manufacturer’s instruction. Random siRNA (siNC) was used as a control. After 48 hours, cells were stimulated with or without TNF-α (10 ng/mL) for 24 hours. Then the cells were lysed and measured with luciferase-reporter assay (Promega) by using a multiwell plate luminometer with an auto-injector (Promega) and analyzed by GloMax 96 Microplate Luminometer Software (Promega), according to the manufacturer’s instruction. Fold changes were calculated for each gene compared with siNC according to the light units.
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