Quickgene 610l dna extraction kit

Genotyping was performed with all patients. Genomic DNA was prepared from leukocytes of peripheral blood with a QuickGene-610L DNA extraction kit (Fujifilm, Tokyo, Japan). During the discovery stage, genome-wide genotyping was performed using the HumanOmni2.5–8 BeadChip Kit (Illumina Inc., San Diego, CA). To ensure high-quality genotype data, a series of quality control filters were applied to the raw data, including a MAF cutoff of >0.01 and genotyping success rate of >95%. Genome-wide imputation was performed using the Michigan Imputation Server (https://imputationserver.sph.umich.edu/index.html) with ASN population haplotypes from the 1000 Genomes project (phase 3) as reference sequences. Series of quality-control filters were applied again to the post-imputed dataset, including a MAF cutoff of >0.01, a genotyping success rate of >90%, and an individual call rate of >90%. Quality control was performed using PLINK (version 1.07; http://pngu.mgh.harvard.edu/~purcell/plink; accessed April 11, 2015). During the replication stage, samples were genotyped using TaqMan SNP assays with the ABI PRISM 7700 system (Applied Biosystems Inc., Foster City, CA). We did not consider deviations in genotype distributions from the Hardy–Weinberg equilibrium because all samples were from patients with AMD.

Genomic DNAs were extracted from peripheral blood leukocytes using QuickGene-610L DNA extraction kit (FUJIFILM Co., Tokyo, Japan). Genotyping was performed using Illumina BeadChip, both OmniExpress and HumanExome, HumanOmni2.5–8, or OmniExpress. The distortion of Hardy-Weinberg equilibrium (HWE) was not considered in this study because all samples comprised AMD cases. Stringent quality control, including minor allele frequency (MAF) ≥ 1% and genotype call rate ≥ 95% (per SNP and per individual), was performed using PLINK ver1.07 (http://pngu.mgh.harvard.edu/~purcell/plink/).