H2O2 (30%) and crystal violet was purchased from Sigma (St. Louis, MO, USA). MTT reagent was purchased from Duchefa Biochemie (Haarlem, Netherlands). anti-pY701-STAT1, anti-STAT1, anti-pY705-STAT3, anti-STAT3, pY694-STAT5, anti-STAT5, anti-pT202/Y204-p44/42 MAPK (Erk1/2), anti-p44/42 MAPK (Erk1/2), anti-pT183/Y185-SAPK/JNK and anti-SAPK/JNK, anti-pT180/Y182-p38, anti-p38, anti-pS473-Akt, anti-Akt, anti-PT389-p70S6 kinase, anti- p70S6 kinase, anti-pS133-CREB, anti-CREB were purchased from Cell Signaling Technology (Danvers, MA, USA). anti-Synaptophysin, anti-PSD95, anti-pT205-Tau, anti-pS262-Tau, and anti-Tau were purchased from ABclonal (Wuhan, China). anti-α-Tubulin was purchased from Abbkine (Wuhan, China). All other chemicals were obtained from Sigma-Aldrich (St. Louis, MO, USA).
Anti py701 stat1
Manufactured by Cell Signaling Technology
Sourced in United States
Anti-pY701-STAT1 is a rabbit monoclonal antibody that recognizes STAT1 phosphorylated at tyrosine 701. It is designed for use in western blotting and immunohistochemistry applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti stat1, by Cell Signaling Technology (4 mentions)
Anti py705 stat3, by Cell Signaling Technology (2 mentions)
Anti stat3, by Cell Signaling Technology (2 mentions)
Anti β actin, by Cell Signaling Technology (2 mentions)
Crystal violet, by Merck Group (1 mentions)
Anti p70 s6 kinase, by Cell Signaling Technology (1 mentions)
Anti p44 42 mapk erk1 2, by Cell Signaling Technology (1 mentions)
Anti akt, by Cell Signaling Technology (1 mentions)
Anti stat5, by Cell Signaling Technology (1 mentions)
Py694 stat5, by Cell Signaling Technology (1 mentions)
Anti pt180 y182 p38, by Cell Signaling Technology (1 mentions)
Anti ps473 akt, by Cell Signaling Technology (1 mentions)
Anti creb, by Cell Signaling Technology (1 mentions)
Anti p38, by Cell Signaling Technology (1 mentions)
Mtt reagent, by Duchefa Biochemie (1 mentions)
Pam3csk4 tlr1 2, by InvivoGen (1 mentions)
Lps tlr4, by InvivoGen (1 mentions)
Anti h pgds, by Cayman Chemical (1 mentions)
Anti cox 2 antibody, by Cayman Chemical (1 mentions)
Mouse ifn γ, by R&D Systems (1 mentions)
4 protocols using anti py701 stat1
1
Evaluating Signaling Pathways in Cells
2
Toll-like Receptor Signaling in Inflammation
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Pam3CSK4 (TLR1/2) and LPS (TLR4) were obtained from InvivoGen (Carlsbad, CA, USA). Mouse recombinant IFN-β was purchased from PBL InterferonSource (now PBL Assay Science, Piscataway, NJ, USA) and mouse IFN-γ was from R&D Systems (Minneapolis, MN, USA). Antibodies used in this study were obtained as follows. Anti-H-PGDS and Anti-COX-2 antibodies were produced by Cayman Chemical (Ann Arbor, MI, USA). Anti-STAT1 and anti-pY701-STAT1 antibodies were from Cell Signaling Technology (Danvers, MA, USA). Anti-actin antibody was from BD Biosciences (Franklin Lakes, NJ, USA). PGs were purchased from Cayman Chemical (Ann Arbor, MI, USA). All other chemicals were from Sigma (St. Louis, MO, USA), unless stated otherwise.
3
Investigating PD-L1 Pathway Regulation
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Sodium bicarbonate (NaHCO3), lactic acid, sodium lactate and sodium oxamate were obtained from Sigma Aldrich (St. Louis, MO, USA). The antibodies used in this study were as follows: anti-PD-L1 (Abcam, Cambridge, UK); anti-LDH-A (Santa Cruz Biotechnology, Santa Cruz, CA, USA); anti-pY701-STAT1, anti-STAT1, anti-pY705-STAT3, anti-pS727-STAT3, anti-STAT3 and anti-β-actin (Cell Signaling Technology, Danvers, MA, USA); and anti-HIF-1α (Abbkine, Wuhan, China).
4
Western Blot Analysis of Signaling Proteins
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Lysates were incubated at 95°C for 10 minutes in Laemmli sample buffer (Bio-Rad Laboratories). Lysates were run on an SDS-PAGE gel with a protein ladder standard (Bio-Rad Laboratories) and transferred to a nitrocellulose membrane (Bio-Rad Laboratories). Blots were incubated in 5% milk in TBST for 1 hour at RT. Blots were incubated in primary antibody (1:1,000 anti-MAPK11 Cell Signaling no. 2339, 1:1,000 anti-MAPK14 Cell Signaling no. 8690, 1:1,000 anti-MAPK12 Cell Signaling no. 2307, 1:1,000 anti-MSK2 Cell Signaling no. 3679, 1:5,000 anti-SARS-CoV-1/2 N (1 g/mL, clone 1C7C7, a kind gift from Thomas Moran), 1:3,000 anti-β-actin Cell Signaling no. 3700, 1:1,000 anti-vinculin Cell Signaling no. 13901, 1:1,000 anti-MX1 abcam no. ab95926, 1:1,000 anti-P(Y701)-STAT1 Cell Signaling no. 9167, 1:1,000 anti-STAT1 Cell Signaling no. 14995) in 1% milk in TBST overnight at 4°C. Blots were washed thrice for 5 minutes in TBST. Blots were incubated in secondary HRP-conjugated (Bio-Rad Laboratories) or infrared-conjugated secondary antibodies (LICOR Biosciences) in 1% milk in TBST. Blots were washed thrice for 5 minutes in TBST. Blots were imaged on a Chemiluminescence digital imager (Bio-Rad Laboratories) using FEMTO ECL reagent (Thermo Fisher Scientific) or an infrared digital imager (LICOR Biosciences).
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