L -NA hydrochloride, ACh chloride, diethylamine NONOate diethyilammonium salt, 8-37 CGRP, rat CGRP, TTX, L -NAME hydrochloride, phentolamine, and suramin were from Sigma-Aldrich, Spain. Stock solutions (10 mmol/L) of drugs were made in distilled water, except for NA, which was dissolved in a NaCl (0.9%)-ascorbic acid (0.01% w/v) solution. These solutions were kept at –20 °C and appropriate dilutions were made in KHS on the day of the experiment.
Rat cgrp
Manufactured by Merck Group
Sourced in Spain
Rat CGRP is a laboratory product used for research purposes. It is a peptide that serves as a neurotransmitter and neuromodulator in the central and peripheral nervous system of rats. The core function of Rat CGRP is to facilitate the study of calcitonin gene-related peptide and its role in physiological and pathological processes in rat models.
Automatically generated - may contain errors
3 protocols using rat cgrp
1
Preparation of Drug Stock Solutions
2
Rat CGRP Injection Protocol
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Rat CGRP (Sigma-Aldrich; 0.5 nmol) and CGRP8-37 (Sigma-Aldrich; 0.5 nmol) were diluted in saline. Isoflurane (1.5–2.0%) was used for brief anesthesia during i.c.v. injections. Drug administration was performed by the direct injection into the right lateral ventricle, through the intact scalp, aiming at 1 mm posterior to bregma and 1 mm right from the midline, as described previously51 (link). H89 (1 mg/kg, Sigma-Aldrich) was administered by intraperitoneal injection, 30 min before CGRP injection.
3
CGRP Receptor Antagonist Injection Protocol
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Rat CGRP (Sigma-Aldrich; 0.5 nmol) and CGRP8-37 (Sigma-Aldrich; 0.5 nmol) were diluted in saline. Isoflurane (1.5-2.0%) was used for brief anesthesia during i.c.v. injections. Drug administration was performed by the direct injection into the right lateral ventricle, through the intact scalp, aiming at 1 mm posterior to bregma and 1 mm right from the midline, as described previously (48) . H89 (1 mg/kg, Sigma-Aldrich) was administered by intraperitoneal injection, 30 min before CGRP injection.
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