Hrp linked anti igg

Manufactured by Cell Signaling Technology

Sourced in United States

HRP-linked anti-IgG is a secondary antibody conjugated with horseradish peroxidase (HRP). It is used to detect and amplify signals from primary antibodies targeting immunoglobulin G (IgG) in various immunoassays, such as western blotting, ELISA, and immunohistochemistry.

Automatically generated - may contain errors

Lab products found in correlation

4 protocols using hrp linked anti igg

Antibody Characterization in Neurobiology

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibodies used in this study are as follows: anti-CNPase (Sigma, St. Louis, MO, USA); anti-APC (Millipore, Billerica, MA, USA); anti-MBP (Millipore); anti-β-tubulin (Sigma); anti-GFP (Abcam, Cambridge, MA, USA); anti-GAPDH (Santa Cruz Biotechnology, Santa Cruz, CA, USA); HRP-linked anti-IgG (Cell Signaling, Danvers, MA, USA); Alexa-Flour568-anti-IgG and Alexa-Flour488-anti-IgG (Molecular Probes). DAPI was purchased from Invitrogen.

Hattori T., Shimizu S., Koyama Y., Emoto H., Matsumoto Y., Kumamoto N., Yamada K., Takamura H., Matsuzaki S., Katayama T., Tohyama M, & Ito A. (2014). DISC1 (Disrupted-in-Schizophrenia-1) Regulates Differentiation of Oligodendrocytes. PLoS ONE, 9(2), e88506.

+ Open protocol

+ Expand

Western Blot Protein Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The anti-Nrf2 (ABclonal, 1 : 2000), anti-Cyclin B1 (Cell Signaling, 1 : 2000), anti-E-cadherin (Cell Signaling, 1 : 2000), anti-Slug (Santa Cruz, 1 : 1000), anti-OCT4 (Cell Signaling, 1 : 2000), anti-Notch1 (Cell Signaling, 1 : 1000), anti-HES-1 (Cell Signaling,1 : 1000), and anti-GAPDH (Santa Cruz, 1 : 5000) were used as primary antibodies. A 1 : 20000 dilution of HRP-linked anti-IgG (Cell Signaling) was used as the secondary antibody. The enhanced chemiluminescent substrate and Western blot film plates from Kodak (Rochester, NY, USA) or Chemidoc MP (Bio-Rad) were used to detect protein levels on the membranes. The density of bands was quantified by ImageJ (National Institutes of Health).

Gong Z., Xue L., Wei M., Liu Z., Vlantis A.C., van Hasselt C.A., Chan J.Y., Li D., Zeng X., Tong M.C, & Chen G.G. (2021). The Knockdown of Nrf2 Suppressed Tumor Growth and Increased the Sensitivity to Lenvatinib in Anaplastic Thyroid Cancer. Oxidative Medicine and Cellular Longevity, 2021, 3900330.

+ Open protocol

+ Expand

Protein Expression Analysis by Western Blot

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Protein extraction and western blot assays were conducted as previously described [15 (link)]. The primary antibodies used in this study were anti-ADAM12 (Proteintech, 1:1000), anti-E-cadherin, anti-N-cadherin, anti-Vimentin, anti-Snail, anti-ERK1/2, anti-phosphorylated-ERK1/2 (all from Cell Signaling Technology, 1:1000), anti-GAPDH (Abclonal, 1:7000), anti-c-Myc (Abcam, 1:1000), anti-EGFR (Abcam, 1:5000), and anti-phosphorylated-EGFR (Abcam, 1:3000). The secondary antibody used here was HRP-linked anti-IgG (Cell Signaling Technology, 1:2000).

Xu J., Wang Y., Jiang J., Yin C, & Shi B. (2023). ADAM12 promotes clear cell renal cell carcinoma progression and triggers EMT via EGFR/ERK signaling pathway. Journal of Translational Medicine, 21, 56.

+ Open protocol

+ Expand

Immunoblotting for p-Stat3 in Lungs

Check if the same lab product or an alternative is used in the 5 most similar protocols

Lungs or 5×10⁶ MC were homogenized and equal amounts of proteins were analyzed by immunoblotting after transfer to nitrocellulose and blocking with 1:1,000 dilutions of anti-p-Stat3 or anti-Stat3 overnight at +4°C. After washes in TBS-0.1%Tween 20, membranes were incubated with 1:5,000 dilution of species-appropriate HRP-linked anti-IgG (Cell Signaling) and sizes of proteins of interest were determined using molecular weight standards (BioRad, Hercules, CA). Quantitation was carried out using ImageJ (National Institutes of Health). Integrated density numbers (in pixels) of p-Stat3 were normalized to total Stat3 and are means of three independent experiments.

Oskeritzian C.A., Hait N.C., Wedman P., Chumanevich A., Kolawole E.M., Price M.M., Falanga Y.T., Harikumar K.B., Ryan J.J., Milstien S., Sabbadini R, & Spiegel S. (2014). The S1P/S1PR2 axis regulates early airway T cell infiltration in murine mast cell-dependent acute allergic responses. The Journal of allergy and clinical immunology, 135(4), 1008-1018.e1.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!