Hpa000694

Manufactured by Merck Group

Sourced in United States

HPA000694 is a laboratory instrument designed for the measurement and analysis of various samples. The core function of this product is to provide accurate and reliable data for scientific research and testing purposes. No further details on the intended use of this product can be provided in an unbiased and factual manner.

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Lab products found in correlation

α tubulin clone b 5 1 2, by Merck Group (1 mentions) Glyceraldehyde 3 phosphate dehydrogenase gapdh, by Merck Group (1 mentions) Immpact dab peroxidase hrp substrate, by Vector Laboratories (1 mentions) Biotin sp affinipure goat anti rabbit igg, by Jackson ImmunoResearch (1 mentions) Peroxidase streptavidin, by Jackson ImmunoResearch (1 mentions) Bz x analyzer software, by Keyence (1 mentions) Bz x710 microscope, by Keyence (1 mentions)

2 protocols using hpa000694

Optimizing Dicer Antibody Detection

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Primary antibodies to Dicer were optimized and used at the following concentrations: mouse monoclonal 13D6 (1:25 for IHC) and 4A6 (made from the same immunogen as 13D6, and selected over 13D6) (1:25 for IHC, and 1:50 for western blot (WB)), rabbit polyclonal ab5818 (1:50 for IHC and WB) (Abcam, Cambridge, MA, USA) (note: ab5818 is currently sold as PA5-19437 ThermoFisher, Grand Island, NY, USA), and rabbit polyclonal HPA000694 (1:50 for IHC, 1:500 for WB) (Sigma-Aldrich, St. Louis, MO, USA). Additional antibodies used for western blot include Topoisomerase I (TOPOI) (C-21) (Santa Cruz Biotechnology Inc., Dallas, TX, USA), α-tubulin clone B-5-1-2, and Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (Sigma).

Spoelstra N.S., Cittelly D.M., Christenson J.L., Gordon M.A., Elias A., Jedlicka P, & Richer J.K. (2016). Dicer Expression in Estrogen Receptor Positive versus Triple-Negative Breast Cancer: An Antibody Comparison. Human pathology, 56, 40-51.

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DICER1 Immunohistochemical Staining Protocol

Cited 1 time

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Immunohistochemical staining for DICER1 was performed using 1:200 polyclonal rabbit anti-DICER1 primary antibody (Sigma-Aldrich HPA000694), and amplification with 1:1,000 Biotin-SP AffiniPure Goat Anti-rabbit IgG and 1:500 peroxidase streptavidin (Jackson ImmunoResearch Laboratories 111-065-144 and 016-030-084, respectively). Samples were developed using ImmPACT DAB peroxidase (HRP) substrate (Vector SK-4105). The rest of the protocol has been detailed elsewhere (19 (link)). Images were acquired using a Keyence BZ-X710 microscope and processed with the BZ-X Analyzer software (Keyence).

Martínez de LaPiscina I., Hernández-Ramírez L.C., Portillo N., Gómez-Gila A.L., Urrutia I., Martínez-Salazar R., García-Castaño A., Aguayo A., Rica I., Gaztambide S., Faucz F.R., Keil M.F., Lodish M.B., Quezado M., Pankratz N., Chittiboina P., Lane J., Kay D.M., Mills J.L., Castaño L, & Stratakis C.A. (2020). Rare Germline DICER1 Variants in Pediatric Patients With Cushing's Disease: What Is Their Role?. Frontiers in Endocrinology, 11, 433.

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