Cysteine, citric acid, PEG200, ε-caprolactone, pyrene, 1, 3-disphenylisobenzofuran (DPBF), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), N-hydroxysuccinimide (NHS) and benzylalcohol were all obtained from Aladdin (Shanghai, China). Enzyme 435, 4-nitrophenyl chloroformate (NPC), cystamine dihydrochloride, and glutathione were provided by J&K Scientific LTD (Beijing, China). Doxorubicin hydrochloride (DOX·HCl) was supplied by Dalian Meilun biotechnology Co., LTD (Dalian, China). Biotin-PEG2000-NH2 (Mw = 2000 Da) was purchased from Shanghai Ponsure Biotechnology Co. Ltd (Shanghai, China). 2′, 7′-dichlorodihydrofluorescein diacetate (DCFH-DA), Annexin V-FITC, propidium iodide (PI), and Hochest 33342 were purchased from Beyotime biotechnology Co., LTD (Shanghai, China). Cypate was synthesized referring to previous work. All other chemicals were obtained from Beijing Chemical Works (Beijing, China) and were used without further purification.
Doxorubicin hydrochloride dox hcl
Manufactured by Meilun
Sourced in China
Doxorubicin hydrochloride (DOX·HCl) is a cytotoxic anthracycline antibiotic commonly used in the treatment of various types of cancer. It is a crystalline solid that is soluble in water and organic solvents. DOX·HCl is a chemotherapeutic agent that inhibits the progression of the cell cycle and induces apoptosis in rapidly dividing cells.
Automatically generated - may contain errors
Lab products found in correlation
4 6 diamidino 2 phenylindole (dapi), by Merck Group (4 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
H2so4, by Tianjin Damao (3 mentions)
Mgcl2, by Tianjin Damao (3 mentions)
Sodium chloride (nacl), by Tianjin Damao (3 mentions)
Hydrochloric acid (hcl), by Tianjin Damao (3 mentions)
Sodium hydroxide (naoh), by Tianjin Damao (3 mentions)
Mitotracker green, by Thermo Fisher Scientific (2 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (2 mentions)
Milli q plus system, by Merck Group (2 mentions)
Penicillin streptomycin solution, by Thermo Fisher Scientific (2 mentions)
Propidium iodide, by Beyotime (2 mentions)
Cystamine dihydrochloride, by J&K Scientific (1 mentions)
Hochest 33342, by Beyotime (1 mentions)
4 nitrophenyl chloroformate, by J&K Scientific (1 mentions)
Glutathione (gsh), by J&K Scientific (1 mentions)
Reduced gsh, by Merck Group (1 mentions)
Sds page gel quick preparation kit, by Beyotime (1 mentions)
Mitochondrial permeability transition pore assay kit, by Beyotime (1 mentions)
Fitc annexin 5 apoptosis detection kit with 7 aad, by BioLegend (1 mentions)
28 protocols using doxorubicin hydrochloride dox hcl
1
Multifunctional Theranostic Platform Development
2
Doxorubicin-loaded Nanoparticle Delivery System
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Doxorubicin hydrochloride (Dox·HCl) was purchased from Dalian Meilun Biotech Co., Ltd. (Dalian, China). GA was purchased from J&K Co. Ltd. (Shanghai, China). Initiator ABIK‐PDS was synthesized according to a previous report. POSS‐NH2 was purchased from Hybrid Plastics (Hattiesburg, USA). Matrigel was purchased from BD Biosciences (Bedford, USA). 3‐(4,5‐dimethyl‐2‐tetrazolyl)‐2,5‐diphenyl‐2H tetrazolium bromide (MTT), 4’,6‐diamidino‐2‐phenylindole (DAPI) and reduced GSH, were purchased from Sigma‐Aldrich (St. Louis, MO, USA). MitoTracker Green was purchased from Invitrogen (Carlsbad, CA, USA). BCA Protein Assay Kit, SDS‐PAGE Gel Quick Preparation Kit, Mitochondrial Permeability Transition Pore Assay Kit, Cell Mitochondria Isolation Kit and Reactive Oxygen Species Assay Kit were purchased from Beyotime Institute of Biotechnology (Shanghai, China). FITC Annexin V Apoptosis Detection Kit with 7‐AAD were purchased from BioLegend. Comonomer HPMA and N‐methacryloylglycylglycyl‐hydrazide‐doxorubicin (MA‐GG‐NHN = Dox) were synthesized according to previous method.[21 ] All other chemicals and reagents were of analytical grade. All other chemicals were bought from Energy Chemical (Shanghai, China).
3
Fabrication and Characterization of Carboplatin-Loaded Nanoparticles
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Carboplatin (CBP) was purchased from Dalian Meilun Biotech Co., Ltd. (Dalian, China). Polylactic acid-Polyethylene glycol (PLA-PEG) was purchased from Shandong Academy of Pharmaceutical Science (Jinan, China). 3-(4, 5-Dimethylthiazol-2-yl)−2,5-diphenyltetrazolium bromide (MTT) was purchased from Solarbio (Shanghai, China). 1,1-dioctadecyl-3,3,3,3-tetramethylindotricarbocyanine iodide (DiR) was procured from Fanbo Biochemicals Co., Ltd (Beijing, China). Fluorescein isothiocyanate (FITC) was purchased from Sigma (MO, USA). DAPI was purchased from Sigma (USA). Doxorubicin hydrochloride (DOX•HCl) was purchased from Dalian Meilun Biotech Co., Ltd. (Dalian, China). Penicillin and streptomycin (1%), trypsin-EDTA (0.25%), phosphate and phosphate buffered saline (PBS) were the product of Solarbio Biotechnology Co. Ltd (Shanghai, China). Roswell Park Memorial Institute medium (RPMI-1640) were obtained from Hyclone. All materials and reagents used in this work were of analytical grade and used without further purification.
4
Multifunctional Nanoparticle Delivery System
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Polyethyleneimine (PEI, branched MW 10 KDa, 99%), celecoxib (CXB, 98%), stearic acid (C18) and N,Nʹ-carbonyldiimidazole (CDI, 98%) were purchased from Energy Chemical Co., Ltd. (Shanghai, China). Doxorubicin hydrochloride (DOX·HCL) was purchased from Meilun Biological Technology (Dalian, China). Bis(2-hydroxyethyl) disulfide, 4-dimethylaminopyridine (DMAP), dimethylmaleic anhydride (DMMA), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC·HCL) and N-hydroxysuccinimide (NHS) were obtained from Mackin Biochemical Co., Ltd. (Shanghai, China). Cells apoptosis assay kit and cells cycle assay kit were purchased from Best Biotechnology Co., Ltd. (Shanghai, China). Primary antibody of P-gp was provide from Solarbio Science & Technology Co., Ltd. (Beijing, China). Human breast cancer cells (MCF-7 and MCF-7/ADR) and lung cancer cells were obtained from KeyGen Biotech (Nanjing, China). Female BALB/c nude mice at the age of 5–6 weeks were purchased from the Cavens Laboratory Animal Limited Company (Changzhou, China). All other chemicals were analytical reagent and used without further purification.
5
Hydrogel-based Drug Delivery System
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The monomers acrylamide (AAm, 98%) and acrylic acid (AAc, AR grade) and the initiator potassium persulfate (KPS, AR grade) were obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China), H2SO4, HCl, NaOH, MgCl2, and NaCl were purchased from Tianjin Damao Chemical Reagent Factory (Tianjin, China), N,N’-Methylenebis(acrylamide) (MBA) was supplied by Shanghai Mackin Biochemical Co., Ltd. (Shanghai, China), doxorubicin hydrochloride (DOX-HCl) was purchased from Dalian MeiLun Biotechnology Co., Ltd. (Dalian, China), and methylene blue (MB) and methyl orange (MO) were purchased from Wenzhou Red Flag Auxiliary Factory (Wenzhou, China). Milli-Q water was used in all experiments.
6
Targeted Doxorubicin Delivery Using DDAT-CTAB Nanoparticles
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Tetrachloroauric acid (HAuCl4) and 2-(Dodecylthiocarbonothioylthio)-2-methylpropionic acid (DDAT) were purchased from Energy Chemical Company (Shanghai, China). Cetyl trimethyl ammonium bromide (CTAB), Acrylamide (AAm), and 2,2′-Azobisisobutyronitrile (AIBN) were obtained from Macklin Reagent Co., Ltd (Shanghai, China). Acrylonitrile (AN), Hydrochloric acid (HCl), L-Ascorbic acid (L-AA) and Methyl thiazolyl tetrazolium (MTT) were provided by Aladdin Reagent Co., Ltd (Shanghai, China). mPEG10k-GPLGIAGQC-SH was synthesized by Shanghai Apeptide Co., Ltd. (Shanghai, China). Doxorubicin hydrochloride (DOX·HCl) was provided by Dalian Meilun Biotechnology Co., Ltd (Liaoning, China). The HepG2 cancer cells were obtained from Nanjing KeyGen Biotech. Co. Ltd. (Nanjing, China). Female nude mice (18–22 g) were purchased from Qinglongshan animal breeding farm (Nanjing, China). All other reagents and solvents were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).
7
Synthesis and Characterization of Anticancer Nanomedicine
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N, N-dimethylformamide (DMF), toluene, triethylamine (TEA), dimethylsulfoxide (DMSO) and dichloromethane (DCM) were dried from CaH2 before use. Hexamethylene diisocyanate (HDI), pyridinium p-toluene sulfonate acid (Py-PTSA), dibutyltin dilaurate (DBTDL), N, N-carbonyldiimidazole (CDI), methoxypolyethylene glycol (mPEG) (Mw=550) and 3-(4,5-dimeth-ylthiazol-2-yl)−2,5-diphenylter-razolium bromide (MTT) were purchased from Sigma-Aldrich (China). Doxorubicin hydrochloride (DOX·HCl) was obtained from Meilun Biological Technology Co, Ltd and desalted with TEA before use. 2,2,2-Trifluoro-N-(2‑methoxy-1,3-dioxolan-4-ylmethyl) acetamide (TA) and N-(1,3-dihydroxypropan-2-yl)−2,2,2-trifluoroacetamide (NF) were synthesis and purified as described previously [24 (link),28] . Human neuroblastoma cancer cell lines (SH-SY5Y), human liver carcinoma cell line (HepG2) and murine hepatic cancer cell line (H22) were obtained from KeyGEN BioTECH (Nanjing, China). Male ICR mice (18–22 g) were purchased from Animal Center of Anhui Medical University (Hefei, China), and used in the Guide of Experimental Animal Ethics Committee of Anhui University.
8
Synthesis and Characterization of Helical Peptides
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α-Helical peptides HPRP-A1 and HPRP-A2 (peptide sequence Ac-FKKLKKLFSKLWNWK-amide, consisting of all L- or all D-amino acids, respectively) were synthesized by solid-phase methods using Fmoc (9-fluorenyl-methoxycarbonyl) chemistry as described previously [20 ] and purified (>95% purity) by RP-HPLC. Further characterization was by mass spectrometry and amino acid analysis. Doxorubicin hydrochloride (DOX·HCl) and epirubicin hydrochloride (EPI·HCl) were purchased from Meilun Biology Technology Co., Ltd. (Dalian, China).
9
Melanoma Cell Targeting with DOX-Loaded GNRs
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Doxorubicin hydrochloride (DOX·HCl) was purchased from Dalian Meilun Biotechnology Co., Ltd. Bovine serum albumin (BSA) was purchased from J&K Scientific Ltd. GNRs, PNIPAM, and PEG-PLA were previously synthesized by our colleagues. Human melanoma cells B16F10 were purchased from the American Type Culture Collection. Dulbecco's Modified Eagle's Medium (DMEM) and fetal bovine serum (FBS) were purchased from Gibco Co, United States. The Cell Counting Kit-8 was purchased from Dojindo Laboratories (Kumamoto, Japan). The apparatus mainly consisted of a UV-VIS spectrophotometer (Cary300, Varian, CA, United States), a freeze dryer (VirTis AdVantage, United States), a dynamic light-scattering device (DLS, ALV/CGS-3, Germany), an infrared light source (IRS-S6, Shanghai, China), a transmission electron microscope (TEM) (Hitachi, H-7000 Electron Microscope), an inverted fluorescence microscope (Olympus IX71, Japan), and an enzyme-labeling instrument (Power Wave XS, Bio-TEK, United States).
10
PLGA-PEG Nanoparticles for Doxorubicin Delivery
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PLGA (MW:20000, 50:50)-PEG (MW: 2000) was purchased from Xi’an Ruixi Biotechnology Co., Ltd (Xi’an, China). IR780 iodide and Poly (vinyl alcohol) (PVA) were obtained from Sigma-Aldrich (USA). Doxorubicin hydrochloride (DOX·HCl) was produced by Dalian Meilun Biotechnology Co., Ltd (Dalian, China). Dichloromethane (CH2Cl2) and isopropanol were purchased from Chongqing Chuandong Chemical Co., Ltd (Chongqing, China). Cell Counting Kit-8 (CCK-8), Calcein AM, and propidium iodide (PI) were purchased from Dojindo (Japan). HMGB1 antibody, CRT antibody, and FITC-conjugated secondary antibody were obtained from Proteintech Group, Inc. (Wuhan, China). ATP-enhanced kit was obtained from Beyotime Biotechnology Co., Ltd. (Shanghai, China). HMGB1, IFN-γ, and TNF-α ELISA Kits were purchased from JingMei Biotechnology Co., Ltd (Shenzhen, China). All chemical agents were used without further purification.
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