Cascade 512b camera

Manufactured by Teledyne

Sourced in United States

The Cascade 512B is a high-performance camera designed for laboratory applications. It features a 512 x 512 pixel sensor and can capture images at a maximum frame rate of 1 kHz. The camera is capable of operating at various gain and exposure settings to accommodate different lighting conditions.

Automatically generated - may contain errors

Lab products found in correlation

Ix81 zdc zero drift microscope, by Teledyne (2 mentions) Eclipse 80i microscope, by Teledyne (1 mentions) Metavue software, by Universal Imaging (1 mentions)

Close spelling variants of this product

Photometrics Cascade 512B CCD camera Cascade 512B CCD camera CASCADE 512B

3 protocols using cascade 512b camera

Hypoxia-Induced Cell Death Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were transfected with NT or BICD1 siRNA for 24 h and then incubated with 0.1 μg/mL of PI-supplied SR media in a live-cell imaging chamber (Tokai, Tokyo, Japan). Normoxic (5% CO₂, 21% O₂, and 74% N₂) or hypoxic (5% CO₂, 0.5% O₂, and 94.5% N₂) gas was supplied to chamber for 48 h. Differential interference contrast images and red fluorescence protein images were acquired over 72 h at 6 h intervals with an Olympus IX81-ZDC zero drift microscope and a Cascade 512 B camera (Roper Scientific, Tucson, AZ, USA). The number of PI-positive cells was determined with the ImageJ software.

Lee H.J., Jung Y.H., Oh J.Y., Choi G.E., Chae C.W., Kim J.S., Lim J.R., Kim S.Y., Lee S.J., Seong J.K, & Han H.J. (2018). BICD1 mediates HIF1α nuclear translocation in mesenchymal stem cells during hypoxia adaptation. Cell Death and Differentiation, 26(9), 1716-1734.

+ Open protocol

+ Expand

Immobilized Cell Microscopy Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were immobilized on agarose pads (1% w/v in reverse osmosis-purified water). Microscopy was performed using a Nikon Eclipse 80i microscope with a PlanApo 100×, 1.40 NA objective and a Cascade 512B camera (Roper Scientific). Images were acquired using Metavue software (Universal Imaging).

Huang C.Y., Gonzalez-Lopez C., Henry C., Mijakovic I, & Ryan K.R. (2020). hipBA toxin-antitoxin systems mediate persistence in Caulobacter crescentus. Scientific Reports, 10, 2865.

+ Open protocol

+ Expand

Hypoxia Impacts on Stem Cell Colonies

Check if the same lab product or an alternative is used in the 5 most similar protocols

After glucosamine or LPA treatment, the culture dish was placed in a temperature and CO₂ control chamber (Tokai, Tokyo, Japan). Normoxic or hypoxic gas was supplied for 48 h. Differential interference contrast images were acquired over those 48 h at 10 min intervals by using an Olympus IX81-ZDC zero-drift microscope and a Cascade 512B camera (Roper Scientific, Tucson, AZ, USA). A constant threshold of each image was maintained. Determination of the mESC colony area was performed by using ImageJ software.

Lee H.J., Ryu J.M., Jung Y.H., Lee K.H., Kim D.I, & Han H.J. (2016). Glycerol-3-phosphate acyltransferase-1 upregulation by O-GlcNAcylation of Sp1 protects against hypoxia-induced mouse embryonic stem cell apoptosis via mTOR activation. Cell Death & Disease, 7(3), e2158-.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!