Ns3694

Manufactured by Merck Group

The NS3694 is a laboratory instrument designed for use in scientific research and analysis. It is a precision device that provides accurate and reliable measurements. The core function of the NS3694 is to perform specific tasks required in laboratory environments, such as data collection and analysis. No further details or interpretation about the intended use of this product are provided.

Automatically generated - may contain errors

Lab products found in correlation

Fk506, by Merck Group (1 mentions) Celastrol, by Merck Group (1 mentions) Z vad fmk, by Selleck Chemicals (1 mentions) Cycloheximide (chx), by Fujifilm (1 mentions) Gentamicin, by Fujifilm (1 mentions) Penicillin, by Fujifilm (1 mentions) Streptomycin, by Fujifilm (1 mentions) Anti apaf 1, by Cell Signaling Technology (1 mentions) Z lehd fmk, by Merck Group (1 mentions) Z ietd fmk, by Merck Group (1 mentions) Anti caspase 9, by Cell Signaling Technology (1 mentions) Anti caspase 3, by Cell Signaling Technology (1 mentions) Hoechst 33258, by Merck Group (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)

3 protocols using ns3694

Tail Regeneration Assay with Drugs

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Tail amputations were carried out as described above and tadpoles were transferred to drug-containing wells immediately after tail amputations. Tadpoles were randomly allocated to drug-containing wells. Drugs were used with following final concentrations unless otherwise stated: 40 µM NS3694 (Sigma-Aldrich, N7787); 100 µM 4-MU (Sigma-Aldrich, M1381); 4.5 µM FK506 (Sigma-Aldrich, F4679); and 50 ng/ml Celastrol (Sigma-Aldrich, C0869). In all experiments, control samples had the same DMSO concentration as that in drug-treated samples (≤0.1% DMSO for single drug treatment and ≤0.2% DMSO in combined drug treatments).

Aztekin C., Hiscock T.W., Butler R., De Jesús Andino F., Robert J., Gurdon J.B, & Jullien J. (2020). The myeloid lineage is required for the emergence of a regeneration-permissive environment following Xenopus tail amputation. Development (Cambridge, England), 147(3), dev185496.

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Isolation and Analysis of Extracellular Vesicles

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Cells were cultured on the 6-well hanging culture insert membrane (Merck Millipore, MCSP06H48) which was kept off the well bottom. After 4 h of treatment with DMSO (Sigma-Aldrich, D2438) (vehicle control), 5 µM rotenone (ICN biomedicals, Inc., 150154) or 10 µM CCCP (Merck Millipore, 215911), inserts containing cell monolayers were removed from the well compartment and conditioned media were collected and ultracentrifuged at 60,000 x g for 1 h. Supernatant was removed and pellet was resuspended in adequate amount of phosphate buffered saline (PBS) (NaCl, KCl, Na₂HPO₄ and KH₂PO₄ in phosphate buffered salts tablets, pH 7.4; Takara Bio, T900) for subsequent analysis. For the assay using apoptosis inhibitors, cells were treated with pan-caspase inhibitor zVAD-FMK (Selleck Chemicals, S7023) 50 µM, tBid inhibitor BI-6C9 (Sigma-Aldrich, B0186) 10 µM and apoptosome inhibitor NS3694 (Sigma-Aldrich, N7787) 50 µM for 4 h before conditioned media were collected.

Choong C.J., Okuno T., Ikenaka K., Baba K., Hayakawa H., Koike M., Yokota M., Doi J., Kakuda K., Takeuchi T., Kuma A., Nakamura S., Nagai Y., Nagano S., Yoshimori T, & Mochizuki H. (2020). Alternative mitochondrial quality control mediated by extracellular release. Autophagy, 17(10), 2962-2974.

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Apoptosis Inhibitors and Assay Methods

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Apoptosis inhibitors, Hoechst 33258, 4′-6-diamidino-2-phenylindole (DAPI), and cell-permeant inhibitors of Apaf-1 (NS3694), caspase-8 (Z-IETD-FMK), caspase-9 (Z-LEHD-FMK), and caspase-3 (Z-EDVD-FMK) were obtained from Sigma-Aldrich (Saint Louis, MO). Fetal calf serum was from Cansera International Inc. (Etobicoke, Canada). STS, gentamicin, penicillin, streptomycin, and cycloheximide were from Wako (Tokyo, Japan). Anti-Apaf-1, anti-caspase-9 and anti-caspase-3 antibodies were from Cell Signaling Technology (Danvers, MA). anti-caspase-9 antibodies were also purchased from Calbiochem (La Jolla, CA) and Abcam (Cambridge, UK). Caspase-9, -8 and -3 Colorimetric Activity Assay kits, and ApopTag Fluorescein kit for TUNEL assays were from Chemicon (Temecula, CA). Chlamydiaceae-specific fluorescein isothiocyanate (FITC)-conjugated monoclonal antibody (Chlamydia-FA) was from Denka Seiken (Tokyo, Japan).

Rahman M.A., Shirai M., Aziz M.A., Ushirokita R., Kubota S., Suzuki H, & Azuma Y. (2015). An epistatic effect of apaf-1 and caspase-9 on chlamydial infection. Apoptosis, 20(10), 1271-1280.

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