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Fl filter set 38 he gfp shift free

Manufactured by Zeiss

The FL Filter Set 38 HE GFP shift free is a high-efficiency fluorescence filter set designed for the detection of green fluorescent protein (GFP) in microscopy applications. The filter set includes an excitation filter, a dichroic beam splitter, and an emission filter, all of which are optimized for the GFP fluorophore. The 'shift free' feature ensures that the excitation and emission wavelengths remain consistent, providing reliable and accurate GFP detection.

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2 protocols using fl filter set 38 he gfp shift free

1

High-Resolution Live-Cell Imaging

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Imaging was performed through a 50× objective (LD EC Epiplan-Neofluar 50×/0.55 DIC) mounted on a Zeiss Axio Examiner D1 microscope. A Zeiss Illuminator HXP 200C light source and an eGFP filter cube (FL Filter Set 38 HE GFP shift free) were used for fluorescence.
An EMCCD camera (Andor iXon Ultra, Oxford Instruments) using Andor Solis software (Oxford Instruments) was used to record videos of 20 s at 512 × 512 pixel resolution. The exposure time was 200 ms (5 Hz).
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2

Fluorescent Antennae Imaging Protocol

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Antennae were imaged through a 10x (Zeiss EC Epiplan-Neofluar 10x/0.25) or a 50x (LD EC Epiplan-Neofluar 50x/0.55 DIC) objective mounted on a Zeiss Axio Examiner D1 microscope. For fluorescence, a light source (Zeiss Illuminator HXP 200C) and eGFP filter cube (FL Filter Set 38 HE GFP shift free) were used.
For image acquisition, an EMCCD camera (Andor iXon Ultra, Oxford Instruments), NIS Elements Advanced Research software (Nikon instruments), and Andor Solis software (Oxford Instruments) were used. Recordings were for 20 seconds, at a resolution of 512×512 pixels, and an exposure time of 200 ms (5 Hz).
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