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0.45 mm nitrocellulose membrane

Manufactured by GE Healthcare
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Sourced in Sweden, United Kingdom

The 0.45-mm nitrocellulose membrane is a laboratory filter designed for filtration and separation applications. It is made of nitrocellulose and has a pore size of 0.45 millimeters. The membrane is used for various filtration tasks in research and clinical settings.

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Lab products found in correlation

Pierce direct ip kit, by Thermo Fisher Scientific (1 mentions) Anti mouse secondary antibody conjugated to horseradish peroxidase, by GE Healthcare (1 mentions) M7 pb e9, by Santa Cruz Biotechnology (1 mentions) Bsep f 6, by Santa Cruz Biotechnology (1 mentions) Supersignal west pico chemiluminescence substrate, by Thermo Fisher Scientific (1 mentions) Anti cdk9 sc 8338, by Santa Cruz Biotechnology (1 mentions) Pierce ecl substrate, by Thermo Fisher Scientific (1 mentions) Uvp chemstudio, by Analytik Jena (1 mentions)

3 protocols using 0.45 mm nitrocellulose membrane

1

CD44 and ITGB1 Immunoprecipitation

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CD44 and ITGB1 were immunoprecipitated from total protein extracts (IP) with anti‐CD44 (EPR1013Y; Abcam) and anti‐ITGB1 (A‐4 clone; Santa Cruz Biotechnology) monoclonal antibodies using Pierce Direct IP Kit (Thermo Scientific) according to the supplier's instructions. Protein samples were separated in reducing SDS/PAGE gels, transferred to 0.45‐mm nitrocellulose membrane (GE Healthcare Life Sciences, Uppsala, Sweden) and blotted for the CD44 and ITGB1, respectively, as well as for STn with TKH2 monoclonal antibody. Protein extracts treated with α‐neuraminidase (Sigma Aldrich) were used as controls.
Cotton S., Azevedo R., Gaiteiro C., Ferreira D., Lima L., Peixoto A., Fernandes E., Neves M., Neves D., Amaro T., Cruz R., Tavares A., Rangel M., Silva A.M., Santos L.L, & Ferreira J.A. (2017). Targeted O‐glycoproteomics explored increased sialylation and identified MUC16 as a poor prognosis biomarker in advanced‐stage bladder tumours. Molecular Oncology, 11(8), 895-912.
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2

Western Blot Analysis of Membrane Proteins

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Membrane proteins (2.5 μg/lane) were separated in a 7.5% sodium dodecyl sulfate polyacrylamide gel and transferred onto a 0.45-mm nitrocellulose membrane (GE Healthcare Life Sciences, Buckinghamshire, UK). The nitrocellulose membrane was incubated overnight at 4°C with a mouse monoclonal anti-BSEP antibody [1:1000 dilution, BSEP (F-6); Santa Cruz Biotechnology, Dallas, TX] and a mouse monoclonal anti-Na+/K+ ATPase antibody (1:1000 dilution, M7-PB-E9; Santa Cruz Biotechnology). Then the nitrocellulose membrane was further incubated for 1 hour at room temperature with an anti-mouse secondary antibody, conjugated to horseradish peroxidase (1: 500; GE Healthcare Life Sciences). The protein–antibody complex was detected by using SuperSignal West Pico Chemiluminescence Substrate (Thermo Fischer Scientific, Vienna, Austria), according to the manufacturer’s recommendations.
Sohail M.I., Schmid D., Wlcek K., Spork M., Szakács G., Trauner M., Stockner T, & Chiba P. (2017). Molecular Mechanism of Taurocholate Transport by the Bile Salt Export Pump, an ABC Transporter Associated with Intrahepatic Cholestasis. Molecular pharmacology, 92(4), 401-413.
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3

Protein Fractionation and Western Blot Analysis

Cited 2 times
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Cytosolic and nuclear protein fractions from VHL or DMSO treated HAP1 cells were extracted as previously described (23 (link),25 (link)). Homogenized samples were heated at 95°C for 5 min, fractioned on SDS-PAGE, transferred onto 0.45 mM nitrocellulose membrane (GE Healthcare Life Sciences), and incubated overnight with anti-PAF1 (D9G9X #12883; 1:2000; Cell Signaling), anti-RTF1 (D7V3W #14737; 1:2000; Cell Signaling), anti-Cdk9 (sc-8338; 1:1000; Santa Cruz), anti-TBP (ab51841; 1:5000; Abcam), anti-Pol II (F-12, sc-55492; 1:1000; Santa Cruz), or anti-FKBP12 (sc-133067; 1:1000; Santa Cruz). Proteins were visualized with Pierce ECL substrate (ThermoFisher Scientific 32106) in a UVP ChemStudio (Analytik Jena).
Santana J.F., Spector B.M., Suarez G.A., Luse D.S, & Price D.H. (2024). NELF focuses sites of initiation and maintains promoter architecture. Nucleic Acids Research, 52(6), 2977-2994.
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