Anti h4k16ac

ChIP assays were performed as described previously36 (link). The following primers were used: sense ChIP-miR-193b-3p F: 5′-GGGAAAAGAGGCTTTTGGAG-3′ and antisense ChIP-miR-193b-3p R: 5′-CCTCACCCTCCCGAGACT-3′. Anti-H3K9ac (Abcam) and anti-H4K16ac (Abcam) antibodies were used to immunoprecipitate chromatin fragments.

The RNA‐FISH probes targeting RP11‐367G18.1 variant 2 were designed using a Stellaris Probe Designer and were purchased from LGC Biosearch Technologies (Table S4). Briefly, cells or tissues were fixed with 4% formaldehyde for 15 min at room temperature, washed, and permeabilized as previously described.¹⁸ Samples were incubated in a hybridization solution with RNA‐FISH probes. Hybridization was performed for 4 h at 55°C avoiding light. Samples were washed for three times, blocked with 1% BSA for 1 h, and subsequently incubated with anti‐HIF‐1α (1:100 dilution, abcam) or anti‐H4K16Ac (1:250 dilution, abcam) antibody at 4°C overnight. Then, samples were washed and incubated with secondary antibody solution (1:500 dilution; goat anti‐rabbit Alexa488, abcam) containing a 1:10000 dilution of the DAPI stock solution for 1 h at room temperature. Images were acquired using a Leica TCS SP8 STED microscope and analyzed using MetaMorph software version 7.8.0.0 (Universal Imaging) and Leica Application Suite X (LAS X version 3.5.5.19976) software. RP11‐367G18.1 variant 2 in cells were counted from three independent replicates.