Agilent whole human genome microarrays 4 44 k

Frozen tissues were shipped on dry ice to Miltenyi Biotec (Bergisch Gladbach, Germany) for microarray analysis. mRNAs were isolated using standard mRNA extraction protocols (Trizol) and were quality-checked via the Agilent 2100 Bioanalyzer platform (Agilent Technologies). The RNA integrity number (RIN) value was calculated and RNA with a RIN number >6 was used [12 (link)]. Agilent Whole Human Genome Microarrays (4 × 44 K) were performed following the manufacturer's protocols. The Rosetta Resolver gene expression data analysis system (Rosetta Biosoftware) was used to compare two single intensity profiles in a ratio experiment (inferior turbinate versus nasal polyp).

For microarray analysis, frozen tissue samples (n = 8) were sent to Miltenyi Biotec (Bergisch Gladbach, Germany). The mRNA was isolated using standard mRNA extraction protocols (Trizol), while the quality was evaluated with the Agilent 2100 Bioanalyzer platform (Agilent Technologies). RNA reaching an RNA integrity number >6 was used [25 (link)]. Agilent Whole Human Genome Microarrays (4×44K) were performed following manufacturer's protocols. Microarray analysis was performed using R version 3.5.2 with limma version 3.38.3 and the Affymetrix Human Genome U95 Set annotation data (Bioconductor package hgu95a.db version 3.8). The heatmap of genes involved in the complement pathways was constructed using the heatmap.2 function as implemented in the gplots package (version 3.0.1).