The standards: asiatic acid (2α,23-dihydroxyursolic acid, 97% purity), arjunolic acid (2α,3β,4α)-2,3,23-trihydroxyolean-12-en-28-oic acid, ≥95 purity), corosolic acid (2α,3β-dihydroxyurs-12-en-28-oic acid, ≥98% purity), maslinic acid (2α,3β-dihydroxyolean-12-en-28-oic acid, ≥98% purity), and (4α)-23-hydroxybetulinic acid ((3β,4α)-3,23-dihydroxylup-20(29)-en-28-oic acid, ≥98% purity) were purchased from Sigma-Aldrich (Spain, Madrid). The standards: madecassic acid (≥90% purity), caulophyllogenin ((3β, 4α, 16α)-3,16,23-trihydroxyolean-12-en-28-oic acid, ≥98.5% purity), and hederagenin ((3β, 4α)-3,23-dihydroxyolean-12-en-28-oic acid, ≥98.5% purity) were purchased from Extrasynthese (France, Paris). The derivatization reagents (pyridine and N,O-bis-(trimethylsilyl)trifluoroacetamide (BSTFA)) and dichloromethane were acquired from Sigma-Aldrich (Spain).
Arjunolic acid
Manufactured by Merck Group
Sourced in United States
Arjunolic acid is a naturally occurring compound that has been isolated from various plant sources. It is a triterpenoid compound with a chemical structure and properties that make it suitable for use in laboratory research and analysis. The core function of arjunolic acid is to serve as a reference standard or analytical tool for researchers and scientists working in fields such as phytochemistry, natural product chemistry, and bioactive compound identification and characterization.
Automatically generated - may contain errors
Lab products found in correlation
Maslinic acid, by Merck Group (2 mentions)
Arjunic acid, by Merck Group (2 mentions)
Arjungenin, by Merck Group (2 mentions)
Arjunetin, by Merck Group (2 mentions)
Dichloromethane, by Merck Group (1 mentions)
Pyridine, by Merck Group (1 mentions)
Corosolic acid, by Merck Group (1 mentions)
Asiatic acid, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
Formaldehyde, by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
Ethidium bromide, by Merck Group (1 mentions)
Naphthylethylenediamine dichloride, by Merck Group (1 mentions)
Hank s balanced salt solution, by HiMedia (1 mentions)
Streptomycin, by HiMedia (1 mentions)
Rpmi 1640, by HiMedia (1 mentions)
Sucrose, by HiMedia (1 mentions)
S aureus atcc 25923, by American Type Culture Collection (1 mentions)
S aureus atcc 29213, by American Type Culture Collection (1 mentions)
S aureus usa300, by American Type Culture Collection (1 mentions)
5 protocols using arjunolic acid
1
Quantification of Pentacyclic Triterpenoids
2
Cytotoxicity Evaluation of Arjunolic Acid
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Arjunolic acid (AA), Ethidium Bromide (EtBr), Acrylidinorage (AO) 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT reagent), fetal bovine serum (FBS), Histopaque 1077, were procured from Sigma (St. Louis, MO, USA). Minimum Essential Medium (MEM), RPMI 1640, sodium chloride (NaCl), sodium carbonate (Na2CO3), penicillin, streptomycin, sucrose, Hanks balanced salt solution, and ethylene diamine tetra acetate (EDTA) were purchased from Himedia, India. NaOH, HCl, Gellatine, formaldehyde, Naphthylethylenediamine dichloride, alcohol and other chemicals were purchased from Merck Ltd., SRL Pvt. Ltd., and Mumbai, India. Commercially available dimethyl sulfoxide (DMSO) was procured from Himedia, India and all other chemicals were from Merck Ltd., SRL Pvt. Ltd. of its highest grade (purity) available.
3
Antibiotic Resistance and Oleanolic Acid Evaluation
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All bacterial strains
are listed inTables S1 and S2 . S. aureus USA300; S. aureus USA400, MRSA 252; S. aureus ATCC
29213; S. aureus ATCC 25904; and S. aureus ATCC 25923 were purchased from American
Type Culture Collection (ATCC). Animal- and human-origin clinical
isolates, including MRSA, E. coli,
and K. pneumoniae, were collected in
Shandong and Jilin, China; these isolates carried one or more β-lactamases.27 (link)S. aureus 8325-4
was obtained from Prof. Timothy J. Foster.15 (link)E. coli BL21(DE3)(pET28a-SP-NDM-1)
carried an NDM-1 gene originating from K. pneumoniae QD-KP2. E. coli BL21(DE3)(pET21a)
was used as a type C β-lactamase-positive strain. In addition, E. coli BL21(DE3)(pET28a) and S. aureus ATCC 25923 were used as negative control strains.
OA and its
analogues (corosolic acid (CA), ursolic acid, maslinic acid, glycyrrhizic
acid, α-boswellic acid, and arjunolic acid) (Figure S1 ) were purchased from Sigma-Aldrich, St. Louis, MO.
All antibiotics were purchased from the National Institute for the
Control of Pharmaceutical and Biological Products (Beijing, China).
are listed in
29213; S. aureus ATCC 25904; and S. aureus ATCC 25923 were purchased from American
Type Culture Collection (ATCC). Animal- and human-origin clinical
isolates, including MRSA, E. coli,
and K. pneumoniae, were collected in
Shandong and Jilin, China; these isolates carried one or more β-lactamases.27 (link)S. aureus 8325-4
was obtained from Prof. Timothy J. Foster.15 (link)E. coli BL21(DE3)(pET28a-SP-NDM-1)
carried an NDM-1 gene originating from K. pneumoniae QD-KP2. E. coli BL21(DE3)(pET21a)
was used as a type C β-lactamase-positive strain. In addition, E. coli BL21(DE3)(pET28a) and S. aureus ATCC 25923 were used as negative control strains.
OA and its
analogues (corosolic acid (CA), ursolic acid, maslinic acid, glycyrrhizic
acid, α-boswellic acid, and arjunolic acid) (
All antibiotics were purchased from the National Institute for the
Control of Pharmaceutical and Biological Products (Beijing, China).
4
HPLC Analysis of Medicinal Triterpenoids
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The pharmacopeial standards of arjunic acid, arjunolic acid, arjungenin, arjunglucoside I, sericic acid and arjunetin were purchased from Sigma Aldrich (St. Louis, MO, USA; p.a.). The organic modifiers for the micellar mobile phase, i.e., acetonitrile and isopropanol, as well as surfactants: polyoxyethylene (23) lauryl ether (Brij35) and dodecyl sodium sulfate (SDS), were purchased from Merck (Darmstadt, Germany; p.a.). The buffer components, i.e., citric acid and disodium hydrogen phosphate (Na2HPO4), were purchased from Sigma Aldrich (Sigma Aldrich, St. Louis, MO, USA; p.a.). Distilled water was obtained from the Direct-Q3 UV apparatus (Millipore, Warsaw, Poland).
5
Quantification of Arjunetin Compounds
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The reference standards of arjunetin (purity, 98.42%), arjungenin (purity, 99.71%), arjunolic acid (purity, 99.0%), and arjunic acid (purity, 99.8%) were procured from Sigma-Aldrich Company, USA. LCMS-grade solvents like acetonitrile and methanol were procured from Honeywell, Germany. Formic acid (MS grade) was procured from Merck, Germany.
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