Apc anti human cd14 antibody

Manufactured by BioLegend

Sourced in United States

The APC anti-human CD14 antibody is a fluorochrome-conjugated monoclonal antibody that recognizes the CD14 antigen on the surface of human cells. CD14 is a glycosylphosphatidylinositol (GPI)-anchored glycoprotein that serves as a co-receptor for the detection of lipopolysaccharide (LPS) and plays a role in the innate immune response. The APC anti-human CD14 antibody can be used in flow cytometry applications to identify and quantify CD14-expressing cells.

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Lab products found in correlation

Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Puromycin, by Merck Group (1 mentions) Lymphoprep density gradient medium, by STEMCELL (1 mentions) Human monocyte isolation kit, by STEMCELL (1 mentions) Fitc anti human cd3 antibody, by BioLegend (1 mentions) Mkc8866, by MedChemExpress (1 mentions) Honokiol, by MedChemExpress (1 mentions) Imq powder, by MedChemExpress (1 mentions) Donkey anti mouse igg h l alexa fluor 647, by Abcam (1 mentions) Anti glyceraldehyde 3 phosphate dehydrogenase gapdh, by Boster Bio (1 mentions) Antibodies against xbp 1s, by Cell Signaling Technology (1 mentions) 3 typ, by MedChemExpress (1 mentions) Lsr 2 flow cytometer, by BD (1 mentions) Fitc anti human flow kit, by BioLegend (1 mentions) Human foxp3 buffer set, by BD (1 mentions) Fc receptor blocking reagent, by Miltenyi Biotec (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Anti human igg fc apc antibody, by BioLegend (1 mentions) Apc antihuman cd3 antibody, by BioLegend (1 mentions) Rosettesep human t cell enrichment cocktail, by STEMCELL (1 mentions)

Close spelling variants of this product

CD14-APC (31 citations) Anti-CD14-APC (13 citations) APC anti-human CD14 (8 citations) Anti-CD14 antibody (3 citations)

5 protocols using apc anti human cd14 antibody

Monocytic Cell Line Responses to OICR-9429 and Immune Complex

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Tohoku Hospital Pediatrics‐1 (THP‐1), a human monocytic cell line, was obtained from Shanghai Institutes of Biological Sciences and maintained in an incubator with 5% CO₂ at 37°C with RPMI 1640 containing 10% foetal bovine serum (Gibco). Transfected cells were selected using puromycin (Sigma–Aldrich). After starving for 16 h, THP‐1 cells were exposed to OICR‐9429 (20 μM, HY‐16993, MedChemExpress) for 24 h and stimulated with the immune complex (IC: β2GPI [100 μg/mL, 11221‐H08H, Sino Biological Inc.]/anti‐β2GPI [10 μg/mL, 11221‐R003, Sino Biological Inc.]) for 4 h for RNA and DNA analyses and 6 h for protein analysis.
Peripheral blood mononuclear cells from three healthy donors (HDs) were extracted using 1.077 g/mL Lymphoprep density gradient medium (StemCell Technologies). APC anti‐human CD14 antibody (301807, BioLegend) and Human Monocyte Isolation Kit (#19359, StemCell Technologies) were used to isolate primary monocytes. After overnight culture, the non‐adherent monocytes were removed. Then, starving cells for 16 h, pretreating with OICR‐9429 for 24 h, and stimulating with IC for 4 h for RNA and DNA analyses and 6 h for protein analysis.

Tan Y., Qiao J., Yang S., Wang Q., Liu H., Liu Q., Feng W., Yang B., Li Z, & Cui L. (2024). ARID5B‐mediated LINC01128 epigenetically activated pyroptosis and apoptosis by promoting the formation of the BTF3/STAT3 complex in β2GPI/anti‐β2GPI‐treated monocytes. Clinical and Translational Medicine, 14(1), e1539.

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Multicolor Immunofluorescence Staining

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Antibodies against XBP1s and Ac-K(Acetylated-Lysine) were obtained from Cell Signaling Technology (Danvers, MA, USA), and anti-SIRT3 antibodies were obtained from Proteintech (Wuhan, China) and Everest Biotech (Oxfordshire, UK). Donkey Anti-Rabbt IgG H&L (Alexa Fluor 488) and Donkey Anti-Mouse IgG H&L (Alexa Fluor 647) were obtained from Abcam (Cambridge, MA, USA). Anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was purchased from Boster Bio (Wuhan, China). FITC anti-human CD3 Antibody, APC anti-human CD14 Antibody were obtained from BioLegend(San Diego, CA). The IMQ powder, MKC8866, 3-TYP and Honokiol were from MedChemExpress (Princeton, NJ, USA). IMQ cream was obtained from Sichuan Med-Shine Pharmaceutical Co., Ltd. (Sichuan, China).

Guo M., Zhuang H., Su Y., Meng Q., Liu W., Liu N., Wei M., Dai S.M, & Deng H. (2023). SIRT3 alleviates imiquimod-induced psoriatic dermatitis through deacetylation of XBP1s and modulation of TLR7/8 inducing IL-23 production in macrophages. Frontiers in Immunology, 14, 1128543.

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Quantification of Intracellular Neutrophil Enzymes

Cited 1 time

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The amounts of intracellular NE and MPO were quantified using a BD LSR II flow cytometer (BD Biosciences, San Jose, CA, USA). Umbilical cord blood (EDTA) was collected immediately after birth (<2 h) as described before [25 (link)] and lysed, and cells were permeabilized in accordance with the instructions of the Human FoxP3 Buffer Set (BD Biosciences). Nonspecific binding was blocked by the Fc Receptor Blocking Reagent (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany). MPO was stained by a FITC-labeled antibody by following the manufacturer’s instructions for the FITC anti-human flow kit (Biolegend, San Diego, CA, USA). For NE staining, cells were incubated with anti-human-NE as a primary antibody (DAKO, Glostrup, Denmark). PE-labeled goat anti-mouse immunoglobulin G (minimal-x-reactivity, Biolegend, San Diego, CA, USA) served as a secondary antibody. Monocytes were identified by APC anti-human CD14 antibody (Biolegend, San Diego, CA, USA). Flow cytometry data were analyzed by FlowJo 8.2.0. The amounts of NE and MPO per cell were quantified by MFI. Isotype and FMO controls were used to determine positive NE/MPO events.

Wirkner A., Vogelgesang A., Hegge I., Lange A., Olbertz D.M., Gerber B., Heckmann M, & Ruhnau J. (2022). Preterm ETs Are Significantly Reduced Compared with Adults and Partially Reduced Compared with Term Infants. Children, 9(10), 1522.

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Generation and Characterization of CD19 CAR T Cells

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CD19 CAR T cells were obtained by frozen donor‐derived CD19 CAR T cells. CAR T cells were manufactured with T cells, obtained through RosetteSep™ Human T Cell Enrichment Cocktail (STEMCELL, Vancouver, CA) and transduced with the lentiviral vector expressing pCDH empty vector (#72266, addgene, MA, USA) which were used as negative control. Dexamethasone (DEX) and Ruxolitinib were purchased from MCE (HY‐50856, MCE USA). Nalm 6 cell line (CD19 positive) was purchased from (CRL‐3273, ATCC, Virginia, USA) and cultured in RPMI 1640 (Gibco, NY, USA) supplemented with 10% FCS. CD19 expression on Nalm 6 cells was confirmed by flow cytometry. Monocytes were isolated from donor peripheral blood mononuclear cells (PBMCs) by multi‐analyte flow assay kit (Human CD8/NK Panel, 740267, BioLegend, San Diego, USA). Anti‐human CD3/CD28 monoclonal antibodies (mAb) were purchased from Stemcell (10971, Stemcell, Vancouver, Canada). Flow cytometry was performed using allophycocyanin (APC)‐anti‐human CD19 antibody (392504, BioLegend, San Diego, USA), APC‐anti‐human CD14 antibody (367117, BioLegend, San Diego, USA), APC‐anti‐human CD3 antibody (300311, BioLegend, San Diego, USA), cell surface expression of CD19 CAR was detected by a recombinant human CD19‐Fc chimera protein (789006, BioLegend, San Diego, USA) and a secondary staining of anti‐human IgG Fc‐APC antibody (409603, BioLegend, San Diego, USA ).

Pan J., Deng B., Ling Z., Song W., Xu J., Duan J., Wang Z., Chang A.H., Feng X, & Tan Y. (2020). Ruxolitinib mitigates steroid‐refractory CRS during CAR T therapy. Journal of Cellular and Molecular Medicine, 25(2), 1089-1099.

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Macrophage Immunophenotyping by Flow

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THP-1-derived macrophages or primary alveolar macrophages collected from ARDS patients were resuspended in 50 µl of PBS. Cell suspension was incubated with 2 µl of APC anti-human CD14 antibody (Biolegend, USA) , FITC anti-human CD68 antibody (Biolegend, USA) and PE anti-human CCR2 antibody (Biolegend, USA) in dark at 4 °C for 30 mins. Flow cytometry analysis was carried out with BD LSRFortessa™ X-20 cell analyzer (BD Biosciences, USA).

Mao Y., Lv X., Xu W., Ying Y., Qin Z., Liao H., Chen L, & Liu Y. (2021). Identification and validation of candidate genes dysregulated in alveolar macrophages of acute respiratory distress syndrome. PeerJ, 9, e12312.

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