Xplore 3d tem tomography software

The serial sections (250 nm) were prepared using a Leica Ultracut UCT or Leica EM UC7 microtome and collected on Formvar-coated copper slot grids. For haltere receptors, cross-sections were collected to facilitate data after processing, e.g., microtubule tracing and alignment. For leg receptors, we collected consecutive lateral sections in most cases because the exoskeleton of fly legs often separated from the embedding resin, and the cross-sections, usually with a small tissue area, had a large chance of getting lost or distorted. Post-staining was performed with 2% uranyl acetate in 70% methanol, followed by 0.4% lead citrate (EMS; 17900). 15-nm gold nanoparticles (BBI Solutions; EMGC15) were added to both sides of the sections as the fiducial markers. The dual-axis tilt series ranging from −60° to 60° were acquired using a FEI Tecnai F30 or FEI Tecnai F20 electron microscope (Thermo Fisher Scientific; formerly FEI). An FEI Tecnai F30 electron microscope was equipped with an Axial Gatan US1000 CCD camera and controlled with SerialEM automated acquisition software (Mastronarde, 2005 (link)). An FEI Tecnai F20 electron microscope was equipped with a Gatan US4000 (895) CCD camera and controlled with FEI Xplore 3D TEM tomography software.