Example 57
Samples of compounds 19-38 were injected on an HPLC system equipped with an Phenomenex Luna C18(2) 5μ, 150×4.6 mm, 100 Å column and eluted with either a gradient of 10% MeCN (0.1% TFA)/90% water (0.1% TFA) to 90% MeCN (0.1% TFA)/10% water (0.1% TFA) or a gradient of 10% MeCN/90% 10 mM potassium phosphate buffer pH 7.4 to 90% MeCN/10% 10 mM potassium phosphate buffer pH 7.4. The retention times obtained for these compounds are depicted in Table 3.
Time Program:
- 0-12 min: 10% MeCN to 90% MeCN
- 12-14 min: 90% MeCN
- 14-15 min: 90% MeCN to 10% MeCN
- 15-17 min: 10% MeCN
TABLE 3
Retention times of compounds 19-23 and 30-38 on RP-HPLC.
retention time (min)
compound0.1% TFApH 7.4
19 (comp.)11.411.4
2011.69.6
2111.69.2
22 (comp.)12.712.6
2310.66.9
30 (comp.)10.510.6
31 (comp.)10.110.1
32 (comp.)9.79.7
3310.49.3
34 (comp.)11.511.5
3511.610.0
3610.49.3
3711.210.5
3811.19.5
US10792369B2. Sulfamide linker, conjugates thereof, and methods of preparation (2020-10-06). Synaffix B.V. [NL]. Inventors: Jorge Merijn Mathieu Verkade [NL], Maria Antonia Wijdeven [NL], Petrus Josephus Jacobus Maria Van De Sande [NL], Sander Sebastiaan Van Berkel [NL], Floris Louis Van Delft [NL].
