Anti tgfβr1

Manufactured by Santa Cruz Biotechnology

Sourced in United Kingdom

Anti-TGFβR1 is a laboratory product that detects the transforming growth factor beta receptor 1 (TGFβR1) protein. It is a tool used in research applications to study the expression and function of TGFβR1.

Automatically generated - may contain errors

Lab products found in correlation

Perfix expose kit, by Beckman Coulter (1 mentions) Cell lysis buffer, by Cell Signaling Technology (1 mentions) Protease inhibitor cocktail, by Merck Group (1 mentions) Anti tgfβr3, by Cell Signaling Technology (1 mentions) Anti tgfβr2, by Abcam (1 mentions) Goat anti rabbit igg conjugated to horseradish peroxidase, by Thermo Fisher Scientific (1 mentions) Anti total smad2 3, by Cell Signaling Technology (1 mentions) Anti phospho smad2, by Cell Signaling Technology (1 mentions) Anti β actin, by Cell Signaling Technology (1 mentions) Supersignal west femto maximum sensitivity substrate, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

TGFβR1 (6 citations)

3 protocols using anti tgfβr1

Characterization of TGF-beta Receptors and JAK2(V617F) Activity

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were washed twice with 1X PBS before getting blocked by 1% bovine serum albumin (BSA) in 1X PBS together with human FC receptor block solution (Biolegend) in 10 minutes at RT. Primary anti-TGFβR1 (Santa Cruz Biotechnology) and anti-TGFβR2 (Invitrogen) antibodies were used and incubated at 4°C for 1 hour and followed by adding FITC-conjugated anti-mouse (for TGFβR1) and anti-rabbit (for TGFβR2) IgG antibodies (Invitrogen) also incubated at 4°C for 1 hour. To detect JAK2(V617F) kinase activity cells were fixed and permeabilized using a PerFix EXPOSE kit according to the manufacturer protocol (Beckman Coulter) followed by staining with anti-phosphotyrosine 4G10 mouse monoclonal antibody conjugated to FITC (Millipore). Cells were washed and re-suspended in 1X PBS before analyzing by flow cytometer and the output was visualized by FlowJo software (BD Biosciences, CA, USA).

Le B.V., Podszywalow-Bartnicka P., Maifrede S., Sullivan-Reed K., Nieborowska-Skorska M., Golovine K., Yao J.C., Nejati R., Cai K.Q., Caruso L.B., Swatler J., Dabrowski M., Lian Z., Valent P., Paietta E.M., Levine R.L., Fernandez H.F., Tallman M.S., Litzow M.R., Huang J., Challen G.A., Link D., Tempera I., Wasik M.A., Piwocka K, & Skorski T. (2020). TGFβR-SMAD3 Signaling Induces Resistance to PARP Inhibitors in the Bone Marrow Microenvironment. Cell reports, 33(1), 108221.

+ Open protocol

+ Expand

Western Blot for ALK5 Protein

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were lysed in 1X cell lysis buffer (Cell Signalling Technologies) supplemented with protease inhibitor cocktail (Sigma) (1/100) and quantified as previously described [19 (link)]. Protein expression was analysed by western blotting after electrophoresis through a 10% SDS polyacrylamide gel as previously described [22 (link)]. Blots were probed for ALK5 protein using anti-TGF-βR1 (Santa Cruz, sc-9048) diluted 1:500 in PBS supplemented with 5% milk overnight at 4°C, washed 3 times for 5 mins per wash in PBS Tween20 (0.05%) and detected with Horseradish peroxidase conjugated anti-rabbit IgG (1:3000 in PBS +5% milk) for 90 mins. Uncropped original blots are shown in S1 and S2 Figs.

Connolly M., Garfield B.E., Crosby A., Morrell N.W., Wort S.J, & Kemp P.R. (2020). miR-1-5p targets TGF-βR1 and is suppressed in the hypertrophying hearts of rats with pulmonary arterial hypertension. PLoS ONE, 15(2), e0229409.

+ Open protocol

+ Expand

Western Blot Analysis of TGFβ Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Protein extracts were prepared from mouse lung tissue and cultured cells as described previously (16) . The following antibodies were employed: anti-TGFβR1 (Santa Cruz Biotechnology, Santa Cruz, CA; 1 : 1,000); anti-TGFβR2 (Abcam, Cambridge, UK; 1 : 500); and anti-TGFβR3 (1 : 100), anti-total-Smad2/3 (1 : 1,000), anti-phospho-Smad2 (phospho-Smad2 Ser465/467; 1 : 1,000), anti-β-actin (1 : 1,000) (all from Cell Signaling, Cambridge, UK). Immune complexes were detected with goat-antirabbit IgG conjugated to horseradish peroxidase (ThermoScientific, Waltham, MA; 1 : 6,000) by enhanced chemiluminescence (SuperSignalWest Femto Maximum Sensitivity Substrate; ThermoScientific).

Rath P., Nardiello C., Surate Solaligue D.E., Agius R., Mižíková I., Hühn S., Mayer K., Vadász I., Herold S., Runkel F., Seeger W, & Morty R.E. (2017). Caffeine administration modulates TGF-β signaling but does not attenuate blunted alveolarization in a hyperoxia-based mouse model of bronchopulmonary dysplasia. Pediatric research, 81(5).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!