α nk1

Manufactured by BioXCell

α-NK1.1 is a laboratory product used for the identification and analysis of natural killer cells. It is a monoclonal antibody that binds specifically to the NK1.1 antigen, which is expressed on the surface of natural killer cells in certain mouse strains. This product can be used in flow cytometry and other immunological assays to detect and study natural killer cells.

Automatically generated - may contain errors

Lab products found in correlation

Asgm1, by Fujifilm (1 mentions) Mouse igg2a, by BioXCell (1 mentions) Rabbit igg, by Southern Biotech (1 mentions) Control clone 2a3, by BioXCell (1 mentions)

2 protocols using α nk1

NK Cell Depletion in Ischemia-Reperfusion Injury

Check if the same lab product or an alternative is used in the 5 most similar protocols

For NK1.1 depleting experiments, B6 and CD1d knock out mice were treated with either 200μg control α-mouse IgG2a (BioXCell C1.18.4, mouse IgG2a Isotype control) or α-NK1.1 (BioXCell PK136) on day −3 and −1. For Asialo-GM1 depleting experiments, B6 mice were treated with 800μg of either rabbit IgG (Southern Biotech) or AsGM1 (WAKO) on day −1. On day 0, mice were then subjected to hanging weight system for 30 minutes to induce IRI. After 1 day of reperfusion, kidney function was measured by GFR. NK cell depletion was verified by measuring NK cell percentages (CD45+CD3−NKp46+).

Victorino F., Sojka D.K., Brodsky K.S., McNamee E.N., Masterson J.C., Homann D., Yokoyama W.M., Eltzschig H.K, & Clambey E.T. (2015). Tissue resident NK cells mediate ischemic kidney injury and are not depleted by anti-Asialo GM1 antibody. Journal of immunology (Baltimore, Md. : 1950), 195(10), 4973-4985.

+ Open protocol

+ Expand

Trabectedin and Immune Checkpoint Blockade

Check if the same lab product or an alternative is used in the 5 most similar protocols

Trabectedin was purchased from PharmaMar (Colmenar Viejo, Madrid, Spain) and dissolved in DMSO to 1 mM (761.8 µg/mL) and kept at −20°C. Therapeutic α-PD-1 (Clone RMT3-23; Catalog#BE0115), α-CD4 (Clone GK1.5; Catalog#:BE0003-1), α-CD8 (Clone 2.43; Catalog#:BE0061), α-NK1.1 (Clone PK136; Catalog#:BE0036), and control (Clone 2A3; Catalog#:BE0089) mAb were purchased from BioXcell (West Lebanon, NH). Antibodies used for flow cytometry were purchased from Tianjing Sungene (Tianjing, China) and eBioscience (San Diego, CA).

Guo Z., Wang H., Meng F., Li J, & Zhang S. (2015). Combined Trabectedin and anti-PD1 antibody produces a synergistic antitumor effect in a murine model of ovarian cancer. Journal of Translational Medicine, 13, 247.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!