Electrophoretic separations of DNA were conducted in gels prepared from agarose (EURx, Gdansk, Poland), Tris-acetate-EDTA buffer (TAE) and ethidium bromide (0.5 µg/mL), using Electrophoresis Sub-Cell and Wide Mini-Sub Cell GT systems (Biorad). The PCR products were visualized under UV light (Gel Doc XR+ gel documentation system, Bio-Rad) and the length of DNA molecules was evaluated against a DNA molecular marker—GeneRuler 100 bp DNA Ladder (Thermo Fisher Scientific), O’GeneRuler 100 bp Plus DNA Ladder (Thermo Fisher Scientific) and Perfect Plus Molecular Weight Quantitative DNA Ladder (EURx). The results were analyzed using Image Lab Software (Biorad), Quantity One 1-D Analysis System (Biorad) and PyElph 1.4 [60 (link)].
O generuler 100 bp plus dna ladder
Manufactured by Thermo Fisher Scientific
Sourced in United States
The O'GeneRuler 100 bp Plus DNA Ladder is a pre-mixed DNA size marker used for sizing and quantifying DNA fragments in agarose gel electrophoresis. It contains DNA fragments ranging from 100 to 3,000 base pairs, with an additional band at 3,000 base pairs.
Automatically generated - may contain errors
2 protocols using o generuler 100 bp plus dna ladder
1
DNA Electrophoresis Separation and Analysis
2
Agarose Gel Electrophoresis and DNA Visualization
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PCR products were loaded on 1% agarose gels containing 100 ng/ml ethidium bromide in Tris-borate-EDTA (TBE) buffer and electrophoresed for 1 h at 150 V. DNA bands were captured under UV light (Quantum-ST4-110/26MX). The sizes of the fragments in a gel were deduced by comparison to O'GeneRuler 100 bp Plus DNA ladder (Thermo scientific, USA).
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