Dionex ultimate 3000 rapid separation rs lc system

The broth extracts were analyzed by high-performance liquid chromatography–high-resolution electrospray ionization-diode array-detector–mass spectrometry (HPLC-HRESI-DAD-MS) on a maXis 4G mass spectrometer (Bruker Daltonics, Billerica, MA, USA) coupled with a Dionex UltiMate 3000 Rapid Separation (RS)LC system (Thermo Fisher Scientific, Waltham, MA, USA) using a BEH C18 column (100 × 2.1 mm, 1.7 μm) (Waters, Eschborn, Germany) with a gradient of 5–95% acetonitrile (ACN) + 0.1% formic acid (FA) in H₂O + 0.1% FA at 0.6 mL/min and 45 °C over 18 min with ultraviolet (UV) detection by a diode array detector (DAD) at 200–600 nm. Mass spectra were acquired from 150 to 2000 m/z at 2 Hz. Detection was performed in the positive MS mode, as more secondary metabolites can be expected to ionize in this mode in comparison to negative ion mode [36 (link),37 ]. The plugin for Chromeleon Xpress (Thermo Fisher Scientific, Waltham, MA, USA, version 6.8) was used for operation of the Dionex UltiMate 3000 RSLC system. HyStar (Bruker Daltonics, Billerica, MA, USA, version 3.2) was used to operate on the maXis 4G mass spectrometer system. HPLC-MS mass spectra were analyzed with DataAnalysis (Bruker Daltonics, Billerica, MA, USA, version 4.2).

The secondary metabolism of broth extracts was analyzed by high-performance liquid chromatography–high-resolution electrospray ionization-diode array-detector–mass spectrometry (HPLC-HRESI-DAD-MS) on a maXis 4G mass spectrometer (Bruker Daltonics, Billerica, MA, USA) coupled with a Dionex UltiMate 3000 Rapid Separation (RS)LC system (Thermo Fisher Scientific, Waltham, MA, USA) using a BEH C18 column (100 × 2.1 mm, 1.7 μm) (Waters, Eschborn, Germany) with a gradient of 5–95% acetonitrile (ACN) + 0.1% formic acid (FA) in H₂O + 0.1% FA at 0.6 mL/min and 45 °C over 18 min with ultraviolet (UV) detection by a DAD at 200–600 nm. Mass spectra were acquired from 150 to 2000 m/z at 2 Hz. Detection was performed in the positive MS mode. The plugin for Chromeleon Xpress (Thermo Fisher Scientific, Waltham, MA, USA, version 6.8) was used for operation of the Dionex UltiMate 3000 RSLC system. HyStar (Bruker Daltonics, Billerica, MA, USA, version 3.2) was used to operate on the maXis 4G mass spectrometer system. HPLC-MS mass spectra were analyzed with DataAnalysis (Bruker Daltonics, Billerica, MA, USA, version 4.2).