Log In Sign up
The largest database of trusted experimental protocols

Mouse beta actin antibody

Manufactured by Merck Group
Visit Supplier

The Merck Mouse Beta-Actin Antibody is a research-use antibody designed to detect and quantify the beta-actin protein in mouse samples. Beta-actin is a widely expressed cytoskeletal protein that is often used as a loading control in Western blot and other protein analysis techniques. This antibody can be used to identify and measure the expression levels of beta-actin in mouse-derived cells or tissues.

Automatically generated - may contain errors

Lab products found in correlation

Clarity ecl kit, by Bio-Rad (1 mentions) Goat anti mouse sc 2031, by Santa Cruz Biotechnology (1 mentions) Proteinase and phosphatase inhibitor cocktail, by Roche (1 mentions)

Close spelling variants of this product

Mouse anti-beta-actin monoclonal antibody (14 citations) Mouse anti-beta-actin antibody (9 citations) Beta-actin mouse monoclonal antibody (4 citations) Mouse monoclonal antibody against beta‐actin (3 citations) Monoclonal mouse anti-beta-actin peroxidase-conjugated IgG primary antibody (2 citations) Mouse anti-beta-actin primary antibody (2 citations) Mouse monoclonal antibody for beta actin (2 citations)

2 protocols using mouse beta actin antibody

1

Immunoblotting Analysis of Ovarian Cell Lines

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunoblotting analysis was
carried out as reported earlier.56 (link) 20 μg
of total cell lysates from various ovarian cell lines were separated
on 10% SDS-PAGE, transferred to PVDF membrane, and detected with antibodies
for PPA1 (Dilution 1:1000; MAB6557, R&D Systems), SMNDC1 (Dilution
1:1000; NBP1–47302, Novus Biologicals), PI15 (Dilution 1:1000;
Clone 3B5, Sigma), and Mouse Beta-Actin antibody (Dilution 1:10000;
A2228 Sigma). HRP-conjugated secondary antibodies (Goat Anti-mouse,
sc-2031 and Goat Anti-rabbit, sc-2030; Santa Cruz Biotechnology) were
used at a dilution of 1:10000. The blots were finally visualized by
chemiluminescence detection using BioRad Clarity ECL kit.
Giri K., Shameer K., Zimmermann M.T., Saha S., Chakraborty P.K., Sharma A., Arvizo R.R., Madden B.J., Mccormick D.J., Kocher J.P., Bhattacharya R, & Mukherjee P. (2014). Understanding Protein–Nanoparticle Interaction: A New Gateway to Disease Therapeutics. Bioconjugate Chemistry, 25(6), 1078-1090.
+ Open protocol
+ Expand
2

Olig1 Knockout Cell Lysate Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total cell lysate was collected from wild type and Olig1-null cells in RIPA buffer (Pierce) supplemented with proteinase and phosphatase inhibitor cocktails (Roche), resolved by SDS-Page and immunoblotted using standard techniques with the antibodies described above in addition to mouse beta-actin antibody (Sigma). Band intensity was measured using ImageJ software (NIH).
Sabo J.K., Heine V., Silbereis J.C., Schirmer L., Levison S.W, & Rowitch D.H. (2017). Olig1 is required for Noggin-induced neonatal myelin repair. Annals of neurology, 81(4), 560-571.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!