Macs bead procedure

Manufactured by Miltenyi Biotec

Sourced in United States

The MACS bead procedure is a cell separation technology developed by Miltenyi Biotec. It utilizes magnetic microbeads coated with antibodies specific to target cell surface markers. These beads bind to the target cells, which can then be separated using a magnetic field. The core function of this procedure is to isolate and purify specific cell populations from complex samples, such as blood or tissue, for further analysis or downstream applications.

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Lab products found in correlation

Ovalbumin (ova), by Merck Group (2 mentions) Ethidium bromide, by Thermo Fisher Scientific (2 mentions) Hema 3 stain kit, by Thermo Fisher Scientific (2 mentions) Tnf α, by R&D Systems (2 mentions) Rpmi 1640 medium, by Merck Group (2 mentions) Tnf α, by Merck Group (1 mentions) Recombinant human ccl11, by R&D Systems (1 mentions)

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MACS beads (115 citations)

2 protocols using macs bead procedure

Eosinophil Isolation and Stimulation

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Granulocytes were isolated from peripheral blood of allergic or healthy donors. Eosinophils were enriched and purified by negative selection using the human eosinophil enrichment cocktail (SSep™, StemCell Technologies, Seattle, WA, USA) and the MACS bead procedure (Miltenyi Biotec, Auburn, CA, USA), as previously described (23 (link)) with the exception that hypotonic red blood cell (RBC) lysis was omitted to avoid any potential for RBC lysis to affect eosinophil function. Eosinophil viability and purity were greater than 99% as determined by ethidium bromide (Molecular Probes, Life Technologies, Carlsbad, CA, USA) incorporation and cytocentrifuged smears stained with HEMA 3 stain kit (Fisher Scientific, Medford, MA, USA), respectively. Purified eosinophils (10⁶ cells/mL) were stimulated with TNF-α (200 ng/mL; R&D Systems, Minneapolis, MN, USA) or recombinant human CCL11 (100 ng/mL; R&D Systems), in RPMI-1640 medium plus 0.1% ovalbumin (Sigma, St. Louis, MO, USA), or medium alone at 37°C, for 1 h. At these concentrations, CCL11 and TNF-α induce consistent cell secretion (16 (link)).

Carmo L.A., Bonjour K., Spencer L.A., Weller P.F, & Melo R.C. (2018). Single-Cell Analyses of Human Eosinophils at High Resolution to Understand Compartmentalization and Vesicular Trafficking of Interferon-Gamma. Frontiers in Immunology, 9, 1542.

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Eosinophil Isolation and Stimulation

Cited 1 time

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Granulocytes were isolated from the blood of different healthy donors. Eosinophils were enriched and purified by negative selection using human eosinophil enrichment cocktail (StemSep™, StemCell Technologies, Seattle WA, USA) and the MACS bead procedure (Miltenyi Biotec, Auburn, CA, USA), as described [57], with the exception that hypotonic red blood cell (RBC) lysis was omitted to avoid any potential for RBC lysis to affect eosinophil function. Eosinophil viability and purity were greater than 99% as determined by ethidium bromide (Molecular Probes, OR, USA) incorporation and cytocentrifuged smears stained with HEMA 3 stain kit (Fisher Scientific, TX, USA), respectively. Experiments were approved by the Beth Israel Deaconess Medical Center Committee on Clinical Investigation, and informed consent was obtained from all subjects. Purified eosinophils (10⁶ cells/mL) were stimulated with TNF-α (200 ng/mL; R&D Systems, USA) or recombinant human CCL11 (eotaxin-1) (100 ng/mL; R&D Systems, Minneapolis, MN) in RPMI-1640 medium plus 0.1% ovalbumin (OVA) (Sigma, St. Louis, MO, USA), or medium alone at 37°C, for 1 h as before [27 (link)].

Carmo L.A., Dias F.F., Malta K.K., Amaral K.B., Shamri R., Weller P.F, & Melo R.C. (2015). Expression and subcellular localization of the Qa-SNARE syntaxin17 in human eosinophils. Experimental cell research, 337(2), 129-135.

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