Cd69 fitc

Manufactured by Miltenyi Biotec

Sourced in Germany

CD69-FITC is a fluorochrome-conjugated monoclonal antibody that binds to the CD69 antigen, an early activation marker expressed on the surface of various immune cell types. It is designed for flow cytometric analysis.

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4 protocols using cd69 fitc

Comprehensive Immune Profiling by Cytofluorimetry

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For cytofluorimetric analysis, cells were stained with surface antibodies in PBS containing 5% FCS for 20 min at 4 °C. The following antibodies were used: IFNγ-PE, CD107a-APC, HLA-DR-PerCP, CD69-FITC, PD-1-APC (Miltenyi Biotec); CD56-PC7, NKp30-PE (Beckman Coulter); NKp46-V450, CD16 PerCP-Cy5,5, CD155-AF647, PD-L1 PE-CF59 (BD Biosciences); E-cadherin-APC (Thermo Fisher Scientific); N-cadherin-PE (Abcam); NKG2D-PE/Dazzle, DNAM1-PE, CD112-PerCP-Cy5,5; MICA/B PC7 (Biolegend); ULBP3 AF405, ULBP2/5/6 PE (R&D).

Fiore P.F., Di Pace A.L., Conti L.A., Tumino N., Besi F., Scaglione S., Munari E., Moretta L, & Vacca P. (2022). Different effects of NK cells and NK-derived soluble factors on cell lines derived from primary or metastatic pancreatic cancers. Cancer Immunology, Immunotherapy, 72(6), 1417-1428.

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Comprehensive Immune Cell Profiling

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PBMCs (treated as above mentioned) were stained with mAb against human CD3 PerCP-Cy5.5 (Biolegend #300327, 5 μL/test) and CD69 FITC (Miltenyi Biotec, 5 μL/test) CD4 PE-Vio770 (Miltenyi Biotec, 2 μL/test), and CD8 APC-Vio770 (Miltenyi Biotec, 2 μL/test). After 30 minutes incubation at 4°C in the dark, cells were washed, centrifuged, and resuspended in staining buffer for the FACS analysis. For each test, cells were analyzed using FACS Verse Flow Cytometer (BD Biosciences).

Ciaglia E., Lopardo V., Montella F., Sellitto C., Manzo V., De Bellis E., Iannaccone T., Franci G., Zannella C., Pagliano P., Di Pietro P., Carrizzo A., Vecchione C., Conti V., Filippelli A, & Puca A.A. (2021). BPIFB4 Circulating Levels and Its Prognostic Relevance in COVID-19. The Journals of Gerontology Series A: Biological Sciences and Medical Sciences, 76(10), 1775-1783.

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Cell Surface Marker Profiling

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Staining of cell surface markers was performed with CD3-APC, CD4-Vioblue, CD8-Viogreen, CD56-PE and CD69-FITC (Miltenyi Biotec). Appropriate isotype-matched controls were used. Cells were examined with BD FACSLyric™ flow cytometer (Biosciences, US) and the data were analyzed with FlowJo 10.6.2.

Najafabadi M.M., Soleimani M., Ahmadvand M., Zomorrod M.S, & Mousavi S.A. (2022). In Vitro Generation of BK polyomavirus-specific T cells for adoptive cell therapy in refractory cystitis hemorrhagic patients after hematopoietic stem cell transplantation. BMC Immunology, 23, 31.

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Multiparameter Flow Cytometry of Lung Cells

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Lung single cells were incubated with fluorescein labeled antibodies, anti-mouse CD11b-PE, CD69-FITC, TLR4-APC, CD49a-PE-vio770, Gr-1-FITC (MACS, Miltenyi, Germany). The cells were analyzed on flow cytometry (BD), and FACS data were analyzed using Cell Quest software (Becton Dickinson).

Li H., Ma L., Li W., Zheng B., Wang J., Chen S., Wang Y., Ge F., Qin B., Zheng X., Deng Y, & Zeng R. (2022). Proline metabolism reprogramming of trained macrophages induced by early respiratory infection combined with allergen sensitization contributes to development of allergic asthma in childhood of mice. Frontiers in Immunology, 13, 977235.

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