Log In Sign up
The largest database of trusted experimental protocols

Qubit br dsdna kit

Manufactured by Agilent Technologies
Visit Supplier

The Qubit BR dsDNA kit is a fluorescence-based assay designed to quantify double-stranded DNA (dsDNA) samples. The kit utilizes a fluorescent dye that binds specifically to dsDNA, allowing for accurate measurement of DNA concentration.

Automatically generated - may contain errors

Lab products found in correlation

High sensitivity, by Agilent Technologies (2 mentions) Nebnext artic sars cov 2 fs kit, by New England Biolabs (2 mentions) Miseq version 2 300 cycle kit, by Illumina (2 mentions) Miseq instrument, by Illumina (2 mentions) Viral rna xpress kit, by Omega Bio-Tek (2 mentions) Tapestation 2200, by Agilent Technologies (2 mentions) Lunascript rt supermix kit, by New England Biolabs (2 mentions)

2 protocols using qubit br dsdna kit

1

Rapid SARS-CoV-2 Genome Sequencing Workflow

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was isolated from VTM within 72 hours of specimen collection using the Omega Bio-Tek Viral RNA Xpress kit (Norcross, GA) on an automated extraction platform. Presence of SARS-CoV-2 RNA was determined by using CDC primers and probes with LunaScript RT Supermix Kit (NEB) run on BioRad (Hercules, CA) C1000 real-time PCR machines. cDNA, PCR amplification, and library preparation was performed using the NEBNext ARTIC SARS-CoV-2 FS kit (NEB) according to the manufacturer’s instructions. Libraries were quantified using Invitrogen Qubit BR dsDNA kit and size determined on an Agilent Tapestation 2200 using high sensitivity d1000 tapes (Santa Clara, CA). Libraries were loaded at 10.5 pM on an Illumina (San Diego, CA) MiSeq version 2, 300 cycle kit on an Illumina MiSeq instrument. Consensus sequences were generated with an in-house data analysis pipeline using bwa, samtools, and ivar.
Kim P.Y., Kim A.Y., Newman J.J., Cella E., Bishop T.C., Huwe P.J., Uchakina O.N., McKallip R.J., Mack V.L., Hill M.P., Ogungbe I.V., Adeyinka O., Jones S., Ware G., Carroll J., Sawyer J.F., Densmore K.H., Foster M., Valmond L., Thomas J., Azarian T., Queen K, & Kamil J.P. (2022). A collaborative approach to improve representation in viral genomic surveillance. bioRxiv.
+ Open protocol
+ Expand
2

Rapid SARS-CoV-2 Genome Sequencing Workflow

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was isolated from VTM within 72 hours of specimen collection using the Omega Bio-Tek Viral RNA Xpress kit (Norcross, GA) on an automated extraction platform. Presence of SARS-CoV-2 RNA was determined by using CDC primers and probes with LunaScript RT Supermix Kit (NEB) run on BioRad (Hercules, CA) C1000 real-time PCR machines. cDNA, PCR amplification, and library preparation was performed using the NEBNext ARTIC SARS-CoV-2 FS kit (NEB) according to the manufacturer’s instructions. Libraries were quantified using Invitrogen Qubit BR dsDNA kit and size determined on an Agilent Tapestation 2200 using high sensitivity d1000 tapes (Santa Clara, CA). Libraries were loaded at 10.5 pM on an Illumina (San Diego, CA) MiSeq version 2, 300 cycle kit on an Illumina MiSeq instrument. Consensus sequences were generated with an in-house data analysis pipeline using bwa, samtools, and ivar.
Kim P.Y., Kim A.Y., Newman J.J., Cella E., Bishop T.C., Huwe P.J., Uchakina O.N., McKallip R.J., Mack V.L., Hill M.P., Ogungbe I.V., Adeyinka O., Jones S., Ware G., Carroll J., Sawyer J.F., Densmore K.H., Foster M., Valmond L., Thomas J., Azarian T., Queen K, & Kamil J.P. (2023). A collaborative approach to improving representation in viral genomic surveillance. PLOS Global Public Health, 3(7), e0001935.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!