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F3165

Manufactured by GeneTex
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The F3165 is a laboratory equipment designed for DNA extraction and purification. It is capable of processing multiple samples simultaneously. The core function of this product is to isolate and purify DNA from a variety of biological samples.

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Lab products found in correlation

Gtx100118, by GeneTex (1 mentions) A6455, by Abcam (1 mentions) Peroxidase conjugated secondary antibody, by Jackson ImmunoResearch (1 mentions) Gtx125989, by Merck Group (1 mentions) A3853, by Merck Group (1 mentions)

2 protocols using f3165

1

Antibody Immunofluorescence Staining

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Antibodies used for the study were as follows: anti-GFP (Molecular Probes, A6455), anti-Atg8 (Abcam, ab109364), anti-Flag (Sigma, F3165), and anti-GAPDH (GeneTex, GTX100118); antibody dilutions as indicated above.
Tang H.W., Spirohn K., Hu Y., Hao T., Kovács I.A., Gao Y., Binari R., Yang-Zhou D., Wan K.H., Bader J.S., Balcha D., Bian W., Booth B.W., Coté A.G., de Rouck S., Desbuleux A., Goh K.Y., Kim D.K., Knapp J.J., Lee W.X., Lemmens I., Li C., Li M., Li R., Lim H.J., Liu Y., Luck K., Markey D., Pollis C., Rangarajan S., Rodiger J., Schlabach S., Shen Y., Sheykhkarimli D., TeeKing B., Roth F.P., Tavernier J., Calderwood M.A., Hill D.E., Celniker S.E., Vidal M., Perrimon N, & Mohr S.E. (2023). Next-generation large-scale binary protein interaction network for Drosophila melanogaster. Nature Communications, 14, 2162.
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2

SDS-PAGE and Western Blotting for Protein Analysis

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Protein samples from cell lysates were incubated at 95°C in Laemmli buffer and subsequently separated by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS–PAGE). Separated protein samples were blotted on a nitrocellulose membrane. MxA/b Mx1, NP or actin levels were determined using specific antibodies against the highly conserved G domain in Mx proteins (M143) [18 (link)], Flag (Sigma-Aldrich, F3165, 1:1000), NP (Gene Tex, GTX125989, 1:1000) and actin (Sigma-Aldrich, A3853; 1:1000), respectively. Primary antibodies were detected using Peroxidase-conjugated secondary antibodies (Jackson ImmunoResearch, 1:5000).
Ciminski K., Pulvermüller J., Adam J, & Schwemmle M. (2019). Human MxA is a potent interspecies barrier for the novel bat-derived influenza A-like virus H18N11. Emerging Microbes & Infections, 8(1), 556-563.
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