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Ab64548

Manufactured by Abcam
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Ab64548 is a lab equipment product manufactured by Abcam. It is a versatile tool designed for scientific research applications.

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Lab products found in correlation

Nanozoomer digital slide scanner, by Hamamatsu Photonics (1 mentions) Leica bond system, by Leica Biosystems (1 mentions) Bond polymer refine detection kit, by Leica Biosystems (1 mentions) Ab6671, by Abcam (1 mentions) Ab59396, by Abcam (1 mentions) Ab78494, by Abcam (1 mentions) Ab21685, by Abcam (1 mentions) Sc 13063, by Santa Cruz Biotechnology (1 mentions) Nbp1 30 894, by Novus Biologicals (1 mentions) Nb600 408, by Novus Biologicals (1 mentions) Sc 7219, by Santa Cruz Biotechnology (1 mentions) Apoptag kit, by Merck Group (1 mentions) Ab46545, by Abcam (1 mentions) Anti bromodeoxyuridine, by Roche (1 mentions) Anti phospho histone h3 ser10, by Merck Group (1 mentions) Anti aurora b, by Merck Group (1 mentions) Anti sarcomeric alpha actinin, by Abcam (1 mentions) Alexa fluor 488, by Thermo Fisher Scientific (1 mentions)

3 protocols using ab64548

1

Quantifying 8-oxoguanine in FFPE Tumor Sections

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Formalin-fixed, paraffin-embedded tumour sections (2.5 μm) were transferred to glass slides. Immunohistochemistry was carried out using the automated Leica BOND system and the Bond Polymer Refine Detection Kit (Leica Biosystems). Go was visualized using a mouse monoclonal 8-oxoguanine primary antibody (ab64548, Abcam, diluted 1:250). The immunostained slides were scanned using the NanoZoomer Digital Slide Scanner (Hamamatsu Photonics).
Giovannini S., Weller M.C., Repmann S., Moch H, & Jiricny J. (2019). Synthetic lethality between BRCA1 deficiency and poly(ADP-ribose) polymerase inhibition is modulated by processing of endogenous oxidative DNA damage. Nucleic Acids Research, 47(17), 9132-9143.
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2

Histological Analysis of Kidney Injury

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Tissue sections were stained with hematoxylin and eosin (HE), Periodic acid-Schiff’s (PAS) and Masson’s trichrome stains using standard protocols. The mesangial area was determined from the PAS stained sections and the fibrotic area from the Masson’s trichrome stained sections as previously described28 (link). The antibodies used in this study were those against FXR (sc-13063, Santa Cruz), Grp78 (ab21685, Abcam), Chop (ab59396, Abcam), 4-hydroxynonenal (4-HNE, ab46545, Abcam), Kim-1 (ab78494, Abcam), 8-oxo-2′-deoxyguanosine (8-oxo-dG, ab64548, Abcam), SDHA (#11998, CellSignaling, Danvers, MA, USA), tumor necrosis factor (TNF, ab6671, Abcam), CD4 (sc-7219, Santa Cruz, Dallas, TX, USA), aSMA (NBP1-30894, Novus Biologicals, Littleton, CO, USA) and Collagen I (ColI, NB600-408, Novus Biologicals). TUNEL staining on kidney paraffin sections was performed with an ApopTag kit (Millipore, Billerica, MA, USA), according to the manufacturer’s instructions.
Gai Z., Chu L., Xu Z., Song X., Sun D, & Kullak-Ublick G.A. (2017). Farnesoid X receptor activation protects the kidney from ischemia-reperfusion damage. Scientific Reports, 7, 9815.
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3

Multiparametric Immunohistochemistry: Cellular Analysis

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Following antigen retrieval with 1mM EDTA in boiling water, sections were blocked with 10% serum from host animal of secondary antibodies, and incubated with primary antibodies overnight at 4°C. Sections were subsequently washed with PBS and incubated with corresponding secondary antibodies conjugated to Alexa Fluor 350, 488 or 555 (Invitrogen). Primary antibodies used are following: anti-phospho Histone H3 Ser10 (Millipore #06–570, 1:100), anti-Aurora B (Sigma A5102, 1:100), anti-Troponin T, Cardiac Isoform Ab-1, Clone 13–11 (Thermo scientific MS-295-P1, 1:100), anti-Bromodeoxyuridine (Roche #11170376001, 1:25), anti-Sarcomeric Alpha Actinin (Abcam, ab68167, 1:100), anti-oxoguanine 8 (Abcam Ab64548, 1:100), anti-phosphorilated ATM (Santa Cruz Biotechnology sc-47739, 1:100), anti-Wee1 (Abcam ab137377, 1:100). Anti-weat germ agglutinin (WGA) conjugated to Alexa Fluor 488 (50 µg/ml, Invitrogen).
Puente B.N., Kimura W., Muralidhar S.A., Moon J., Amatruda J.F., Phelps K.L., Grinsfelder D., Rothermel B.A., Chen R., Garcia J.A., Santos C.X., Thet S., Mori E., Kinter M.T., Rindler P.M., Zacchigna S., Mukherjee S., Chen D.J., Mahmoud A.I., Giacca M., Rabinovitch P.S., Aroumougame A., Shah A.M., Szweda L.I, & Sadek H.A. (2014). The Oxygen Rich Postnatal Environment Induces Cardiomyocyte Cell Cycle Arrest Through DNA Damage Response. Cell, 157(3), 565-579.
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