Yeast cells were cultured to mid-log phase, chromatin was extracted as described previously (38 (link)), whole-cell extracts were prepared by TCA methods. The proteins were separated by SDS-PAGE, and tranferred to Immun-Blot PVDF Membranes (BIO-RAD). 1× TBST (50 mM Tris pH 7.5, 150mM NaCl, 0.05% Tween 20) diluted primary and secondary antibodies were incubated overnight at 4°C and 1 h at room temperature respectively, the membranes were imaged by Amersham Imager 680 (GE Healthcare). Antibodies used of western blot: Anti-acetyl-Histone H4 antibody (Millipore: 06-598), Histone H4 antibody (Active Motif: 61521), Anti-Histone H2A.Z antibody (Millipore: 07–718), Anti-α-Tubulin antibody (Sigma-Aldrich: T6199), anti-myc antibody is prepared by our laboratory.
Histone h4 antibody
Manufactured by Active Motif
Histone H4 antibody is a laboratory reagent used to detect and study the histone H4 protein, which is a core component of nucleosomes in eukaryotic cells. This antibody can be used for various applications, such as Western blotting, immunoprecipitation, and immunocytochemistry, to investigate the presence, distribution, and modifications of histone H4.
Automatically generated - may contain errors
Lab products found in correlation
Amersham imager 680, by GE Healthcare (1 mentions)
Immun blot pvdf membrane, by Bio-Rad (1 mentions)
Anti acetyl histone h4 antibody, by Merck Group (1 mentions)
Anti α tubulin antibody, by Merck Group (1 mentions)
Amersham imager, by Cytiva (1 mentions)
Sp1 sc 14027, by Santa Cruz Biotechnology (1 mentions)
Strep hrp antibody 71591, by Merck Group (1 mentions)
Ha antibody mms 101p, by Fortrea (1 mentions)
3 protocols using histone h4 antibody
1
Histone Modification Analysis in Yeast Cells
2
Antibody Catalog for Protein Interaction Studies
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Normal rabbit IgG (sc-2027), RNA polymerase II (sc-899x), CCNT1 (sc-8127 and sc-10750), MEPCE (sc-82542), and SP1 (sc-14027) were purchased from Santa Cruz Biotechnology. AFF1 (A302-344A), AFF4 (A302-538A), UBE2O (A301-873A), Ku70 (A302-624A-T), and SFPQ (A301-322A-T) antibodies were purchased from Bethyl. The HA antibody (MMS-101P) was purchased from Covance. LARP7 antibodies were purchased from Aviva (ARP40847_P050) and Santa Cruz (sc-515209). HEXIM1 antibodies were purchased from Abcam (ab25388) and Everest (EB06964). CDK9 antibodies were purchased from Santa Cruz (sc-8338 and sc13130) and ProMab (30212). The tubulin (T6199) and FLAG (F1804) antibodies were purchased from Sigma. The RNA pol II phosphor-Ser2 (ab5095) was purchased from Abcam. The STREP-HRP antibody (71591) and IgG (12-371) were purchased from Millipore. The histone H4 antibody (39269) was purchased from Active Motif.
3
Histone H4 and CaBdf1 Regulation in C. albicans
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C. albicans strains were grown at 30 °C in SC media to an OD600 of 0.5–0.8. Cells were lysed in FastprepTM (MPBiologicals) twice at 6.5 m s−1 for 1 min with intermediate incubation on ice. Histone H4 antibody was purchased from Active Motif (ref. 39269) and the CaBdf1 antibody was developed in house using a full-length recombinant protein injected in rabbits (Covalab).
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