Zag enzyme linked immunosorbent assay elisa kit

In the groups without ascites and with ascites but without treatment, serum samples were collected when patients presented at the hospital. In the group with ascites and treatment, serum collection was performed immediately before the administration of tolvaptan. Albumin (g/dL), total bilirubin (mg/dL), sodium (mEq/L), creatinine (mg/dL), BUN (mg/dL), CRP (mg/dL), α-fetoprotein (AFP; ng/mL) and des-γ-carboxy prothrombin (DCP; mAU/mL) were measured. Serum samples were kept at -80 °C until copeptin (pmol/L), ZAG (μg/dL), cystatin C (mg/dL), NGAL (ng/mL) and L-FABP (ng/mL) measurements were performed using an automated copeptin immunofluorescent assay kit (Thermo Fisher Scientific Inc., Tokyo, Japan), ZAG enzyme-linked immunosorbent assay (ELISA) kit (BioVendor, Brno, Czech Republic), cystatin C ELISA kit (R&D Systems, Minneapolis, MN, USA), NGAL ELISA kit (R&D Systems) and high-sensitivity human L-FABP ELISA kit (CMIC Holdings, Tokyo, Japan), respectively.
The Child-Pugh score, albumin-bilirubin (ALBI) score (18 (link)), fibrosis index based on 4 factors (FIB-4) (19 (link)), model for end-stage liver disease (MELD) score, MELD-Na score and estimated glomerular filtration rate (eGFR) with Cockcroft and Modification of Diet in Renal Disease (MDRD) formula were calculated.

Blood samples were collected when patients arrived at the hospital. Albumin (g/dL), cholinesterase (ΔPH), total bilirubin (mg/dL), aspartate transaminase (AST, U/L), alanine aminotransferase (ALT, U/L), γ-glutamyltransferase (γ-GT, U/L), alkaline phosphatase (ALP, U/L), blood urea nitrogen (BUN, mg/dL), creatinine (mg/dL), sodium (mEq/L), C-reactive protein (CRP, mg/dL), platelet counts (×10⁴/μL), prothrombin time (%), ammonia (μg/dL), total cholesterol (mg/dL), triglyceride (mg/dL), blood sugar (mg/dL), hemoglobin A1c (HbA1c, %), branched-chain amino acid (μmol/L) and tyrosine (μmol/L) were retrospectively collected from patient hospital records. Serum samples were kept at -80°C until ZAG (μg/dL) measurements, using a ZAG enzyme-linked immunosorbent assay (ELISA) kit (BioVendor, Brno, Czech Republic). The AST-to-platelet ratio index (APRI), fibrosis index based on 4 factors (FIB-4) (9 (link)), albumin-bilirubin (ALBI) score (10 (link)), Child-Pugh score, model for end-stage liver disease (MELD) score, serum branched-chain amino acid to tyrosine ratio (BTR) and estimated glomerular filtration rate (eGFR) with Cockcroft formula were calculated.