Blood samples were collected and transported to the laboratory, where they were stored at -80 °C until use. Fibulin-1 levels were measured in ethylenediaminetetraacetic acid-treated plasma diluted 1:500 using the Circulex immunoassay kit on a Triturus 4-Plate platform (Grifols, Barcelona, Spain) according to the manufacturer’s instructions. The intra-assay and inter-assay coefficients of variation were < 8 % and < 12 % at a level of 10 µg/mL, respectively. The detection limit of the assay was 0.9 µg/mL, and serum samples were diluted in parallel to standard curve. The results were not available to the clinicians during the study.
Triturus 4 plate platform
Manufactured by Grifols
Sourced in Spain
The Triturus 4-Plate platform is a laboratory instrument designed for automated sample processing. It features four independent sample plates and can perform various liquid handling tasks, such as pipetting, mixing, and incubation. The Triturus 4-Plate platform is a versatile tool that can be used in various laboratory applications.
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Lab products found in correlation
2 protocols using triturus 4 plate platform
1
Fibulin-1 Levels in Plasma
2
Therapeutic Drug Monitoring of TNF-inhibitors
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The trough level of drugs and ADA was measured using ELISA kits (Promonitor-ADL #5080230000; Promonitor-Anti-ADL #5090230000; Promonitor-IFX #5060230000; Promonitor-anti-IFX #5070230000; Promonitor-ETN #5110230000; Promonitor-anti-ETN#5120230000; Promonitor-GLM #5200230000 and Promonitor-anti-GLM #5210230000) (Progenika, Derio, Spain) on a Triturus 4-Plate platform (Grifols, Barcelona, Spain). Drug concentrations were expressed in mg/L, and cut-off values for ADA in arbitrary units according to the manufacture’s instruction, except for anti-IFX (>2 AU/mL). The cut-off value for anti-IFX were adjusted to the detection limit as the initial validation showed clearance of IFX at this level. Between patient variability was calculated based on the first TDM TNFi through level in all patients. The within patient variability were calculated in the subgroup of patients that had more than three consecutive TNFi trough level measurements and were not changed in treatment during the one year TDM measurement period. TDM algorithms for responders (proactive) and non-responders (reactive) are shown in Figure 1 .
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